Scholarlink

Lasy-Seq (low-cost and easy RNA-seq) is a high-throughput library preparation method for 3′ RNA-seq. Usage of oligo(dT) primers with unique index sequences allows simultaneous reverse transcription (RT) reaction and sample indexing. Therefore, each post-RT step, which was performed on a sample-by-sample basis in a conventional method, can be performed on a large number of samples together in a single tube. With the Lasy-Seq, the sequencing-ready library pool can be prepared from a few to several hundred samples in a few days.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Lasy-Seq: A High-Throughput 3′ RNA-Seq Method for Large-Scale Transcriptome Profiling in Rice

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Natsumi Mori-Moriyama,
  • Atsushi J. Nagano

摘要

Lasy-Seq (low-cost and easy RNA-seq) is a high-throughput library preparation method for 3′ RNA-seq. Usage of oligo(dT) primers with unique index sequences allows simultaneous reverse transcription (RT) reaction and sample indexing. Therefore, each post-RT step, which was performed on a sample-by-sample basis in a conventional method, can be performed on a large number of samples together in a single tube. With the Lasy-Seq, the sequencing-ready library pool can be prepared from a few to several hundred samples in a few days.