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Actinorhizal plant genome function has been systematically explored in a new way, thanks to recent advances in genome engineering, which have been fuelled by the CRISPR-associated RNA-guided endonuclease Cas9. Using a brief RNA guide string, Cas9 may be accurately guided to particular regions within complicated genomes, much like the search feature in contemporary word computers. Almost any selected organism can benefit from this system’s simple editing or modification of DNA sequences found in its endogenous genome and their functional consequences. In this chapter, we give a crop genome editing pipeline with best practices and step-by-step instructions for improving chances of success. The goal of this study was to examine the CRISPR gene prediction on Frankia genome.

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Genome Engineering of Frankia sp.

  • 章节

  • 发表日期：

    2025年

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  • Radhamanalan Guhanraj,
  • Dharumadurai Dhanasekaran

摘要

Actinorhizal plant genome function has been systematically explored in a new way, thanks to recent advances in genome engineering, which have been fuelled by the CRISPR-associated RNA-guided endonuclease Cas9. Using a brief RNA guide string, Cas9 may be accurately guided to particular regions within complicated genomes, much like the search feature in contemporary word computers. Almost any selected organism can benefit from this system’s simple editing or modification of DNA sequences found in its endogenous genome and their functional consequences. In this chapter, we give a crop genome editing pipeline with best practices and step-by-step instructions for improving chances of success. The goal of this study was to examine the CRISPR gene prediction on Frankia genome.