Scholarlink

Genetic information of plant microbiome is an important approach to explore the relationship between plants and microorganisms. PCR amplification and high-throughput sequencing technology can more accurately reveal the species and genetic diversity of microbial communities. In this chapter, we extracted plant genomes using the modified CTAB method, PCR amplification of plant endophytic fungi using 18S and ITS region primers, and high-throughput sequencing and analysis. Through this method, the information of microbial community structure of endophytic fungi allowed us to obtain.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

DNA Extraction, Amplification, and High-Throughput Sequencing of the 18S and ITS Regions of Endophytic Fungi

  • 章节

  • 发表日期：

    2025年

查看全文
  • Yuqing Niu,
  • Yingjie Liu,
  • Peng Chen

摘要

Genetic information of plant microbiome is an important approach to explore the relationship between plants and microorganisms. PCR amplification and high-throughput sequencing technology can more accurately reveal the species and genetic diversity of microbial communities. In this chapter, we extracted plant genomes using the modified CTAB method, PCR amplification of plant endophytic fungi using 18S and ITS region primers, and high-throughput sequencing and analysis. Through this method, the information of microbial community structure of endophytic fungi allowed us to obtain.