Excipient emulsions improve the bioavailability and bioaccessibility of carotenoids. Excipient emulsions are fabricated using an emulsifier, oil, and aqueous phase. The mean particle diameter, particle size distribution, electrical charge, microstructure, and lycopene bioaccessibility after simulated digestion are evaluated for the carotenoid loaded emulsions. Carotenoid bioaccessibility depends on the type and level of lipids, emulsifiers and microfluidizer parameters (the number of passes and pressure) used to form the excipient emulsions which is attributed to differences in the solubilization capacity of mixed micelles formed from different lipid digestion products. In general, the incorporation of excipient emulsion can offer a novel approach to enhance the bioaccessibility of carotenoids. This emulsion integrates an aqueous phase, which typically includes carotenoid or carotenoid-rich sources, and a lipid phase composed of a hydrophobic substance or its solution in an appropriate solvent. Emulsion homogenization is a two-step process starting with the development of a primary, coarse emulsion, followed by the formation of a fine emulsion. The emulsion quality, alongside the encapsulation efficacy, is significantly influenced by the initial mean droplet size and the physical stability of emulsions. Furthermore, the bioavailability of the encapsulated carotenoids, indicated by their digestibility, serves as a critical metric for assessing the effectiveness of emulsion-based encapsulation methods.