Simultaneous recording from multiple brain regions is essential for understanding large-scale interactions among distributed neural networks. Recent advances in recording techniques have brought about increased knowledge of how different brain regions cooperate in various cognitive processes. Here, we describe a protocol combining widefield calcium imaging with electrical recordings using a flexible, insertable, and transparent microelectrode probe (Neuro-FITM). This multimodal approach allows simultaneous recording of neural activity from the dorsal cortex and subcortical brain regions in awake, head-fixed mice. This protocol provides guidelines on how to implement this technique, including surgical preparation, recording setup, and data processing.