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Neurotransmitters (NTs) and neuromodulators (NMs) play vital roles in various physiological and pathological processes. To better understand the transmission mechanisms of NTs or NMs (NTs/NMs), it is critical to monitor the dynamics of NTs/NMs with high sensitivity, specificity, and spatiotemporal resolution. With recent advances in genetically encoded NT/NM indicators (GENIs) and mesoscopic imaging, it is now possible to visualize the spatiotemporal dynamics of NTs/NMs in vivo on a large scale, even cover the entire mouse neocortex, enabling simultaneous recording of multiple brain regions to further investigate the relationship among different cortex regions. Here, we present an overview of the principle, development, and application of GENIs, focusing on their joint utilization with mesoscopic imaging.

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Mesoscopic Imaging of Neurotransmitters and Neuromodulators with Genetically Encoded Sensors

  • 实验方案

  • 发表日期：

    2025年

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  • Fei Deng,
  • Jiesi Feng,
  • Hao Xie,
  • Yulong Li

摘要

Neurotransmitters (NTs) and neuromodulators (NMs) play vital roles in various physiological and pathological processes. To better understand the transmission mechanisms of NTs or NMs (NTs/NMs), it is critical to monitor the dynamics of NTs/NMs with high sensitivity, specificity, and spatiotemporal resolution. With recent advances in genetically encoded NT/NM indicators (GENIs) and mesoscopic imaging, it is now possible to visualize the spatiotemporal dynamics of NTs/NMs in vivo on a large scale, even cover the entire mouse neocortex, enabling simultaneous recording of multiple brain regions to further investigate the relationship among different cortex regions. Here, we present an overview of the principle, development, and application of GENIs, focusing on their joint utilization with mesoscopic imaging.