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Yeast two-hybrid (YTH) technology is a powerful tool for studying protein interactions and has been widely used in various fields of molecular biology, including the study of antiviral innate immunity. This chapter presents detailed information and experimental procedures for identifying virus-host protein interactions involved in immune regulation using yeast two-hybrid technology.

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Yeast Two-Hybrid Assay for Investigating Antiviral Innate Immunity

  • 实验方案

  • 发表日期：

    2025年

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  • Sisi Xia,
  • Xiaoping Huo,
  • Chunfu Zheng,
  • Jun Chen

摘要

Yeast two-hybrid (YTH) technology is a powerful tool for studying protein interactions and has been widely used in various fields of molecular biology, including the study of antiviral innate immunity. This chapter presents detailed information and experimental procedures for identifying virus-host protein interactions involved in immune regulation using yeast two-hybrid technology.