In the last decades, we have witnessed the worldwide spread and growing impact of one of the most important neglected tropical diseases, the dengue fever. Even though it continues to be neglected mainly due to its major impact on the more socio and economically vulnerable populations there was progress toward a more complete understanding about the basic biology of dengue infection in mosquitoes and humans as well as translational research to develop antivirals and improved vaccines. Paradoxically, dengue fever incidence has steadily grown globally suggesting that the development/refinement of basic/translational research and control approaches are not keeping the pace of dengue spread. Therefore, in this last chapter, we will discuss the latest developments regarding preparedness and response against dengue, looking into their applicability to reduce dengue fever around the globe.