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Precise tracking of transplanted cells within mouse tissues is essential for evaluating engraftment, migration, and long-term persistence in transplantation and regenerative medicine research. Here, we present a practical and versatile cell-tracking workflow focusing on PKH26-based direct fluorescent labeling and GFP-mediated indirect genetic labeling. PKH26 provides rapid and robust membrane labeling that is useful for short-term tracing, while GFP enables stable and heritable fluorescence suitable for long-term monitoring of transplanted cells. This chapter provides optimized protocols, troubleshooting strategies, and critical technical considerations for implementing these complementary methods. Together, these approaches offer a flexible and reliable framework for evaluating the engraftment, survival, distribution, and behavior of transplanted cells in mouse models.

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Tracking of Transplanted Stem Cells in Mouse Models Using Practical Fluorescence-Based Protocols

  • 实验方案

  • 发表日期：

    2026年

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  • Riho Kanai,
  • Arvind Hariharan,
  • Janaki Iyer,
  • Younan Liu,
  • Takashi I,
  • Yoshinori Sumita,
  • Simon D. Tran

摘要

Precise tracking of transplanted cells within mouse tissues is essential for evaluating engraftment, migration, and long-term persistence in transplantation and regenerative medicine research. Here, we present a practical and versatile cell-tracking workflow focusing on PKH26-based direct fluorescent labeling and GFP-mediated indirect genetic labeling. PKH26 provides rapid and robust membrane labeling that is useful for short-term tracing, while GFP enables stable and heritable fluorescence suitable for long-term monitoring of transplanted cells. This chapter provides optimized protocols, troubleshooting strategies, and critical technical considerations for implementing these complementary methods. Together, these approaches offer a flexible and reliable framework for evaluating the engraftment, survival, distribution, and behavior of transplanted cells in mouse models.