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Patient-derived organoids (PDOs) preserve the architecture and heterogeneity of primary tumors and enable clinically relevant ex vivo testing. We describe standardized protocols for tumor tissue dissociation, extracellular-matrix embedding, tumor-type-specific medium formulation, serial passaging, and histologic and genomic evaluation, alongside quality control criteria to ensure viability and reproducibility across major solid cancers. These methods support precision oncology, drug screening, and translational studies using living biobanks of PDOs.

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Patient-Derived Cancer Organoids: Standardized Protocols for Tumor Cell Isolation, Organoid Generation, and Serial Passaging Across Multiple Cancers

  • 实验方案

  • 发表日期：

    2025年

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  • Ali Sahin,
  • Dila Sener-Akcora,
  • Ayse Mine Yilmaz,
  • Muharrem Okan Cakir,
  • Betul Karademir-Yilmaz

摘要

Patient-derived organoids (PDOs) preserve the architecture and heterogeneity of primary tumors and enable clinically relevant ex vivo testing. We describe standardized protocols for tumor tissue dissociation, extracellular-matrix embedding, tumor-type-specific medium formulation, serial passaging, and histologic and genomic evaluation, alongside quality control criteria to ensure viability and reproducibility across major solid cancers. These methods support precision oncology, drug screening, and translational studies using living biobanks of PDOs.