Negative magnetophoresis is employed to levitate cells in a paramagnetic medium without the need for magnetic labeling, preserving their natural state and minimizing toxicity. The single-ring magnet configuration that provides an open space in the levitation chamber enhances culture accessibility and scalability, enabling the formation of millimeter-sized 3D structures through cellular self-assembly. This system provides a versatile and cost-effective approach for diverse applications, including tissue engineering and biofabrication. This protocol outlines a method for biofabrication and maintenance of 3D cellular structures using magnetic levitation with a ring magnet-based setup.