Scholarlink

Mass spectrometry-based techniques have emerged as a gamechanger for the analysis of carbohydrates. Here, we summarize state-of-the-art glycan analysis in vacuo, demonstrating how mass spectrometry, tandem mass spectrometry, ion mobility spectrometry, and gas-phase infrared spectroscopy, supported by chromatography methods and computational modelling, facilitate the structural characterization of isolated glycans and the analysis of the entire glycome. After an introduction to glycans and techniques, the sugar classes of N-glycans, O-glycans, human milk oligosaccharides, and glycosaminoglycans will be discussed in detail, before the chapter ends with an outlook on the future of mass spectrometry-based glycan analysis.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Advanced Mass Spectrometry Techniques for the Characterization of Carbohydrates

  • 章节

  • 发表日期：

    2025年

查看全文
  • Niklas Geue,
  • Caitlin Walton-Doyle,
  • Eleonora Renzi,
  • Mathew Bejoy,
  • Kevin Pagel

摘要

Mass spectrometry-based techniques have emerged as a gamechanger for the analysis of carbohydrates. Here, we summarize state-of-the-art glycan analysis in vacuo, demonstrating how mass spectrometry, tandem mass spectrometry, ion mobility spectrometry, and gas-phase infrared spectroscopy, supported by chromatography methods and computational modelling, facilitate the structural characterization of isolated glycans and the analysis of the entire glycome. After an introduction to glycans and techniques, the sugar classes of N-glycans, O-glycans, human milk oligosaccharides, and glycosaminoglycans will be discussed in detail, before the chapter ends with an outlook on the future of mass spectrometry-based glycan analysis.