<p class="MsoNormal"><span lang="EN-CA" style="mso-ascii-font-family: Aptos; mso-fareast-font-family: Aptos; mso-hansi-font-family: Aptos; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-CA;">This second edition volume expands on the previous edition with new protocols that offer biochemical, biophysical, and biological assays for studying macrophage migration inhibitory factor (MIF) and D-dopachrome tautomerase (D-DT). The chapters in this book cover topics such as generation of MIF peptides via solid phase peptide synthesis; fragment screening of MIF by surface plasmon resonance; MIF NMR chemical shift perturbation mapping; methods to determine the role of MIF in cilia biogenesis; and immunofluorescent quantification of MIF in larval zebrafish tailfin transfection.&#xa0;</span><span style="mso-ascii-font-family: Aptos; mso-fareast-font-family: Aptos; mso-hansi-font-family: Aptos; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US;">Written in the highly successful <em>Methods in Molecular Biology</em> series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.<br></br></span><span lang="EN-CA" style="mso-ascii-font-family: Aptos; mso-fareast-font-family: Aptos; mso-hansi-font-family: Aptos; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-CA;"><br></br></span><span style="mso-ascii-font-family: Aptos; mso-fareast-font-family: Aptos; mso-hansi-font-family: Aptos; mso-bidi-font-family: 'Times New Roman'; mso-ansi-language: EN-US;">Cutting-edge and comprehensive, <em>Macrophage Migration Inhibitory Factor: Methods and Protocols, Second Edition </em>is a valuable resource for any researcher hoping to contribute to the ever expanding world of MIF biology.</span></p>