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Abstract <p>Gold nanoparticles are widely used in spectroscopy for detecting/studying substances in biological media. Such particles in biological objects inevitably interact with proteins, which obviously affects the functions and properties of the nanomaterial. The influence of external conditions on the interaction of nanoparticles with proteins in the aqueous phase has still remained little studied. In this paper we investigate the influence of temperature and pH of the medium on the interaction of lysozyme protein with gold nanoparticles in aqueous solutions. It is found that the temperature of the medium affects the size of aggregates of gold nanoparticles with protein at pH 7.5 of the aqueous solutions. It is found that lysozyme prevents protein aggregation at acidic pH values; with an increase in temperature, gold particles with a lysozyme protein crown retain their size. The results of this study gain a deeper insight into the processes of interaction between enzymes and gold nanoparticles with a change in the solvent properties.</p>

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Investigation of the Influence of pH and Temperature of an Aqueous Solution on the Stability of the Gold Nanoparticles–Lysozyme Protein System by Optical Methods

  • 期刊论文

  • 发表日期：

    2025年6月1日

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  • E. A. Molkova,
  • T. A. Matveeva,
  • I. V. Baimler,
  • R. M. Sarimov,
  • S. V. Gudkov,
  • A. S. Dorokhov,
  • A. Yu. Izmailov

摘要

Abstract

Gold nanoparticles are widely used in spectroscopy for detecting/studying substances in biological media. Such particles in biological objects inevitably interact with proteins, which obviously affects the functions and properties of the nanomaterial. The influence of external conditions on the interaction of nanoparticles with proteins in the aqueous phase has still remained little studied. In this paper we investigate the influence of temperature and pH of the medium on the interaction of lysozyme protein with gold nanoparticles in aqueous solutions. It is found that the temperature of the medium affects the size of aggregates of gold nanoparticles with protein at pH 7.5 of the aqueous solutions. It is found that lysozyme prevents protein aggregation at acidic pH values; with an increase in temperature, gold particles with a lysozyme protein crown retain their size. The results of this study gain a deeper insight into the processes of interaction between enzymes and gold nanoparticles with a change in the solvent properties.