Abstract <p>The study was conducted to obtain doubled haploids with the <i>Pi-b</i> gene for breeding the rice <i>Oryza sativa</i> L. As a result of hybridization between the Far Eastern varieties and Oxy 2x sample (carrier of the <i>Pi-b</i> gene), we have created 13 F₁ hybrids of six hybrid combinations: Sadko × Oxy 2x, Lugovoy × Oxy 2x, Darius 23 × Oxy 2x, Almaz × Oxy 2x, doubled haploid (Don 4237 × Dolinny) × Oxy 2x, and Khankaisky 429 × Oxy 2x. In vitro androgenesis technology was used to transfer the original F₂ donor plants to a constant homozygous state. Thirty-five hybrid plants carrying the resistance allele of the <i>Pi-b</i> gene were preliminarily selected. Ten thousand two hundred forty-six rice anthers were introduced into the in vitro culture. Callus formation was observed in 25 of the 35 F₂ hybrids. The highest values of callus formation have been recorded in the Lugovoy × Oxy 2x and Khankaisky 429 × Oxy 2x hybrid plants (9.33 and 27.50%, respectively); on average, it was low for the entire sample (2.45%), which is nontypical for the Far Eastern genotypes. Regeneration was observed on the calli of seven rice hybrids of four hybrid combinations: Darius 23 × Oxy 2x, Sadko × Oxy 2x, Khankaisky 429 × Oxy 2x, and Lugovoy × Oxy 2x. Based on the results of PCR analysis, 33 plants with the resistance allele of the <i>Pi-b</i> gene were selected from the 63 rice doubled haploids. Forty-four lines of doubled haploids were sown on a vegetation plot; three of them have not sprouted. The Oxy 2x collection sample with a caryopsis strongly attached to the panicle axis produces a phenotype with varying degrees of shattering in the homozygous state in doubled haploids when interacting with the loci of other varieties responsible for the strength of caryopsis attachment: 11 lines with moderate shattering and 30 lines with strong shattering. Thus, the total yield of the initial breeding material is very limited for subsequent selections under field conditions and unpromising due to caryopsis shattering.</p>