Scholarlink

<p>The goal of this study is to identify the effect of deletion of some genes encoding biogenesis of outer membrane protein complex components on the virulence of <i>Yersinia pseudotuberculosis</i> mutants. <i>Y.&#xa0;pseudotuberculosis</i> <i>surA</i>, <i>degP</i>, <i>bamB</i>, and <i>ompH</i> mutants were generated by mutagenesis using suicide vectors. The constructed mutants were characterized by growth rate, sensitivity to antibiotics, bile salts, and sodium dodecyl sulfate, as well as to the bactericidal action of normal human serum. The total composition of the outer membrane proteins of the knockout mutants was determined by SDS-PAGE. The virulence of Δ<i>surA</i>, Δ<i>degP</i>, Δ<i>bamB</i>, and Δ<i>ompH</i> was studied after subcutaneous, intraperitoneal, or oral administration of mice. It was found that BamB, OmpH, and SurA proteins were necessary for maintaining the integrity of the <i>Y. pseudotuberculosis</i> cell membrane and that SurA and BamB proteins are important for resistance to the bactericidal action of serum complement, and a high degree of attenuation of <i>Y. pseudotuberculosis</i> strains with deleted <i>surA</i>, <i>degP</i>, and <i>bamB</i> genes for outbred mice was revealed. The <i>surA</i>, <i>degP</i>, and <i>bamB</i> genes can be used as potential molecular targets for construction of <i>Y. pseudotuberculosis</i> vaccine strains.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Disruption of Outer Membrane Biogenesis Leads to Attenuation of Yersinia pseudotuberculosis

  • 期刊论文

  • 发表日期：

    2025年6月1日

查看全文
  • A. S. Trunyakova,
  • R. Z. Shaikhutdinova,
  • T. V. Gapel’chenkova,
  • A. S. Vagaiskaya,
  • N. A. Lipatnikova,
  • E. A. Krasil’nikova,
  • E. M. Mazurina,
  • T. I. Kombarova,
  • S. V. Dentovskaya,
  • A. P. Anisimov

摘要

The goal of this study is to identify the effect of deletion of some genes encoding biogenesis of outer membrane protein complex components on the virulence of Yersinia pseudotuberculosis mutants. Y. pseudotuberculosis surA, degP, bamB, and ompH mutants were generated by mutagenesis using suicide vectors. The constructed mutants were characterized by growth rate, sensitivity to antibiotics, bile salts, and sodium dodecyl sulfate, as well as to the bactericidal action of normal human serum. The total composition of the outer membrane proteins of the knockout mutants was determined by SDS-PAGE. The virulence of ΔsurA, ΔdegP, ΔbamB, and ΔompH was studied after subcutaneous, intraperitoneal, or oral administration of mice. It was found that BamB, OmpH, and SurA proteins were necessary for maintaining the integrity of the Y. pseudotuberculosis cell membrane and that SurA and BamB proteins are important for resistance to the bactericidal action of serum complement, and a high degree of attenuation of Y. pseudotuberculosis strains with deleted surA, degP, and bamB genes for outbred mice was revealed. The surA, degP, and bamB genes can be used as potential molecular targets for construction of Y. pseudotuberculosis vaccine strains.