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Abstract <p>A new strain of the unicellular green microalga <i>Micractinium thermotolerans</i> VKM Al-430 (Trebouxiophyceae, Chlorophyta) was isolated from a technogenically polluted reservoir. Its taxonomic affiliation was determined by analyzing the sequences of internal transcribed spacer regions ITS1 and ITS2 and by comparing their secondary structures with those of the authentic strain <i>M. thermotolerans</i> VKM Al-332. In a series of intensive-cultivation experiments in a modern photobioreactor under elevated carbon dioxide concentration (up to 5%), the new strain demonstrated remarkable biomass productivity, reaching a maximum cultivation density of 6.3 g/L (dry weight; 6.2 g/L according to the optical density sensor, ODS), with total lipid content of 11–12% of dry weight. Furthermore, it exhibited near-complete consumption of phosphate and nitrate components from the culture medium. The fatty acid profile of the biomass was dominated by palmitic, oleic, linoleic and α-linolenic acids. These results make this rapidly growing strain a promising candidate for various applications in modern microalgal biotechnology.</p>

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A Novel Strain of the Microalga Micractinium thermotolerans VKM Al-430: Growth and Biomass Accumulation under Intensive Cultivation Conditions

  • 期刊论文

  • 发表日期：

    2026年7月20日

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  • P. N. Scherbakov,
  • A. Y. Shaenko,
  • E. S. Krivina,
  • A. D. Temraleeva,
  • A. A. Krupatina

摘要

Abstract

A new strain of the unicellular green microalga Micractinium thermotolerans VKM Al-430 (Trebouxiophyceae, Chlorophyta) was isolated from a technogenically polluted reservoir. Its taxonomic affiliation was determined by analyzing the sequences of internal transcribed spacer regions ITS1 and ITS2 and by comparing their secondary structures with those of the authentic strain M. thermotolerans VKM Al-332. In a series of intensive-cultivation experiments in a modern photobioreactor under elevated carbon dioxide concentration (up to 5%), the new strain demonstrated remarkable biomass productivity, reaching a maximum cultivation density of 6.3 g/L (dry weight; 6.2 g/L according to the optical density sensor, ODS), with total lipid content of 11–12% of dry weight. Furthermore, it exhibited near-complete consumption of phosphate and nitrate components from the culture medium. The fatty acid profile of the biomass was dominated by palmitic, oleic, linoleic and α-linolenic acids. These results make this rapidly growing strain a promising candidate for various applications in modern microalgal biotechnology.