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Abstract <p>The contact printing method is widely used to create microchips and develop highly sensitive biosensors. This method is based on techniques for immobilizing biomolecules. In this work, two effective methods for covalent immobilization of oligonucleotides based on reactions of disulfide bond formation and azide–alkyne cycloaddition are optimized for the contact printing method. With both immobilization strategies, oligonucleotides retain the ability to hybridize with complementary DNA, which provides a basis for their practical use in bioanalytical applications.</p>

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Optimization of Covalent DNA Immobilization for Contact Printing Method

  • 期刊论文

  • 发表日期：

    2025年4月1日

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  • A. A. Titov,
  • A. E. Kuznetsov,
  • N. V. Komarova

摘要

Abstract

The contact printing method is widely used to create microchips and develop highly sensitive biosensors. This method is based on techniques for immobilizing biomolecules. In this work, two effective methods for covalent immobilization of oligonucleotides based on reactions of disulfide bond formation and azide–alkyne cycloaddition are optimized for the contact printing method. With both immobilization strategies, oligonucleotides retain the ability to hybridize with complementary DNA, which provides a basis for their practical use in bioanalytical applications.