<p>The mechanical characterization of natural stone under varying moisture conditions is a critical issue for structural assessment, particularly when only non-destructive techniques are feasible. This study aims to characterize the Arroyo del Toro limestone and to establish reliable relationships between dynamic and static elastic moduli for application in finite element modelling. A multiscale experimental programme was conducted on 19 cylindrical specimens extracted from different quarry locations and depths. Physical properties, mineralogical composition and microstructural features were analysed, and mechanical tests were performed under controlled moisture conditions (0%, 40% and 100%) to determine dynamic and static elastic moduli and uniaxial compressive strength.&#xa0;The limestone is classified as a biosparite composed of more than 96% calcite, with an open porosity of approximately 17%. Results show a pronounced moisture-dependent degradation of mechanical performance, mainly between dry and partially saturated states, with reductions of approximately 21% in dynamic modulus, 28% in static modulus and 46% in uniaxial compressive strength. A strong empirical correlation between dynamic and static elastic moduli was obtained (<InlineEquation ID="IEq1"> <EquationSource Format="TEX">\(R^2 = 0.95\)</EquationSource> <EquationSource Format="MATHML"><math> <mrow> <msup> <mi>R</mi> <mn>2</mn> </msup> <mo>=</mo> <mn>0.95</mn> </mrow> </math></EquationSource> </InlineEquation>).&#xa0;The derived correlations enable the reliable estimation of static elastic modulus from non-destructive dynamic measurements. This provides a practical framework for defining mechanical input parameters in finite element modelling, improving the accuracy of structural assessment while minimizing the need for destructive testing.</p>