Abstract <p>This study introduces a novel approach combining low-voltage electron microscopy (LVEM) with graphene liquid cells (GLCs) for high-contrast, high-resolution imaging of hydrated samples in their original state. LVEM, operating below 30 kV, reduces beam damage and enhances contrast, particularly for low atomic weight materials, without the need for staining. This dual technique enables real-time observation of dynamic processes. Organic and inorganic nanoparticles were imaged to demonstrate the effectiveness of the technique. It offers superior contrast and better signal-to-noise ratios for imaging samples in liquid environments, compared to the results obtained from conventional high-voltage electron microscopy.</p> Graphical abstract <p></p>