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<p>In this work, microspheres coated with Au–Ag nanostars (Nss-Sph) were synthetized and evaluated by SERS with methylene blue (MB) and bovine serum albumin (BSA). The system was evaluated by UV–Vis absorption of the Nss-Sph, where changes in the wavelengths and absorption were observed compared to the Nanostars (Nss) spectrum. The composition of the Nss and Nss-Sph was evaluated by Raman spectroscopy where some characteristic vibrational modes of MPA, EDC and NHS were observed. SEM micrographs show the morphology of Nss, while the Nss-Sph were formed by adding the MPA-EDC-NHS molecules. EDS showed changes in the atomic percentages of gold and silver in the Nss-Sph systems. Finally, the Nss-Sph were evaluated with MB and BSA by SERS measurements.</p> Graphical abstract <p></p>

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Detection of MB and BSA with Au–Ag nanostar-coated microspheres

  • 期刊论文

  • 发表日期：

    2025年1月29日

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  • Josué I. García Ramírez,
  • Marlen D. Méndez Castillo,
  • Erick O. Santos Santiago,
  • Julián Hernández Torres,
  • Adriana Báez Rodríguez,
  • Enrique Juárez Aguilar,
  • Irma Y. Izaguirre Hernández,
  • Pablo Thomas Dupont,
  • Luis Zamora Peredo

摘要

In this work, microspheres coated with Au–Ag nanostars (Nss-Sph) were synthetized and evaluated by SERS with methylene blue (MB) and bovine serum albumin (BSA). The system was evaluated by UV–Vis absorption of the Nss-Sph, where changes in the wavelengths and absorption were observed compared to the Nanostars (Nss) spectrum. The composition of the Nss and Nss-Sph was evaluated by Raman spectroscopy where some characteristic vibrational modes of MPA, EDC and NHS were observed. SEM micrographs show the morphology of Nss, while the Nss-Sph were formed by adding the MPA-EDC-NHS molecules. EDS showed changes in the atomic percentages of gold and silver in the Nss-Sph systems. Finally, the Nss-Sph were evaluated with MB and BSA by SERS measurements.

Graphical abstract