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<p>This research presents the design and fabrication of 3D scaffolds composed of a chitin polymeric matrix, hydroxyapatite, collagen, and almond membrane reinforcement for applications in tissue engineering. To evaluate their performance, scaffolds were developed and characterized in two distinct compositions. Chitin was chemically extracted from <i>Lentinula edodes</i> (shiitake mushrooms), hydroxyapatite was synthesized via the chemical coprecipitation method using chicken eggshells as a precursor, collagen was extracted from the inner membrane of the eggshell, and almond membranes were included for their notable antioxidant capacity. Each component, as well as the scaffolds developed, was characterized using SEM and FTIR techniques, along with biocompatibility and biodegradation tests in simulated body fluids.</p> Graphical abstract <p></p>

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Design and fabrication of scaffolds composed of hydroxyapatite, chitin, almond membrane and collagen for tissue engineering

  • 期刊论文

  • 发表日期：

    2025年1月6日

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  • C. Vázquez-Padilla,
  • G. Carbajal-De la Torre,
  • M. Estévez,
  • N. N. Zurita-Méndez,
  • M. A. Espinosa-Medina

摘要

This research presents the design and fabrication of 3D scaffolds composed of a chitin polymeric matrix, hydroxyapatite, collagen, and almond membrane reinforcement for applications in tissue engineering. To evaluate their performance, scaffolds were developed and characterized in two distinct compositions. Chitin was chemically extracted from Lentinula edodes (shiitake mushrooms), hydroxyapatite was synthesized via the chemical coprecipitation method using chicken eggshells as a precursor, collagen was extracted from the inner membrane of the eggshell, and almond membranes were included for their notable antioxidant capacity. Each component, as well as the scaffolds developed, was characterized using SEM and FTIR techniques, along with biocompatibility and biodegradation tests in simulated body fluids.

Graphical abstract