Scholarlink

<p>An automated layer-by-layer (LbL) technique for creating extracellular matrix (ECM) mimetic microenvironments was developed using heparin and collagen coatings on cell culture microplates. By leveraging a liquid-handling robot, we achieved consistent and uniform coating deposition across multiple wells. Surface characterization confirmed coating homogeneity, and cell studies demonstrated significant improvements in cell viability. Human Schwann cells and mesenchymal stem cells showed approximately 160–170% increased viability compared to uncoated surfaces. The coatings remained stable for five months, highlighting the method's robustness. This approach offers a scalable, standardized platform for studying cell-material interactions in an ECM-like environment.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Optimizing automated layer-by-layer deposition of engineered ECM-like microenvironments for mammalian cell culture

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月29日

查看全文
  • Luis Pinzon-Herrera,
  • Ian Popp,
  • Roaa Hadi,
  • Jorge Almodovar

摘要

An automated layer-by-layer (LbL) technique for creating extracellular matrix (ECM) mimetic microenvironments was developed using heparin and collagen coatings on cell culture microplates. By leveraging a liquid-handling robot, we achieved consistent and uniform coating deposition across multiple wells. Surface characterization confirmed coating homogeneity, and cell studies demonstrated significant improvements in cell viability. Human Schwann cells and mesenchymal stem cells showed approximately 160–170% increased viability compared to uncoated surfaces. The coatings remained stable for five months, highlighting the method's robustness. This approach offers a scalable, standardized platform for studying cell-material interactions in an ECM-like environment.

Graphical Abstract