<p>Recent advancements in 3D cellular morphology open new possibilities for <i>in vitro</i> monitoring that better reflect the complexity of real tissue environments. To support this, the development of electrode systems featuring 3D architectures is essential. In this study, we engineered inkjet-printed 2D-to-3D transforming electrodes capable of organizing 3D neuronal cells. The electrodes were implemented as electrochemical dopamine sensors enabling spatially resolved sensing in 3D spheroidal cells. This study demonstrates a cleanroom-free, flexible-substrate fabrication approach that provides a promising platform for advanced bioanalytical studies in complex 3D cell systems.</p> Graphical abstract <p></p>