<p>The scientific community has traditionally believed that cells of the same type are identical and exhibit homogeneous behavior. However, it is now well understood that the behavior of a single cell may differ significantly from that of its surrounding population. This understanding of single-cell heterogeneity has led to numerous advances in the fields of biology and medicine. Despite these advances, there is still a lack of reliable single-cell analysis platforms. In this work, we propose a magnetic microfluidic platform that allows users to capture single, magnetically labeled cells at discrete locations and allows for the release of any desired cell. We experimentally demonstrate the capture of superparamagnetic beads and individual magnetically labeled Jurkat cells at discrete locations and their subsequent release. With its advantages over existing devices, our platform has the potential to further facilitate single-cell analyses and support developments in fields, such as cancer, stem cell, and neurobiology research.</p> Graphical Abstract <p></p>