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<p>A triaxial nanofiber membrane was developed using a custom triaxial nozzle during electrospinning. The membrane consisted of three layers outer (PVA-ZnO), middle (PVA-Collagen/Type I-BMP-2), and inner (PVA-nHAP). TEM revealed the diameters of the outer, middle, and inner layers as 167.5, 142.6, and 119.3&#xa0;nm, respectively. The triaxial nanofiber membrane exhibited superior tensile strength (36&#xa0;MPa) and elastic modulus (0.025 GPa), along with a roughness of 359&#xa0;nm and hydrophilic properties. In addition, it demonstrated a 200% swelling ratio and reduced bacterial colonization by 10.8-fold. Both Calcium deposition and ALP activity were significantly higher and increased expression of BSP (eightfold), OCN (13-fold), and OPN (488-fold) genes were observed in membrane + OST groups (p &lt; 0.05), compared to MSC and OST groups. These findings highlight that the membrane has potential for enhance bone repair by combining well-structured, antimicrobial, mechanical, and osteoinductive properties.</p> Graphical Abstract <p></p>

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Characterization of a new triaxial nanofiber membrane and osteogenic differentiation capacity of human bone marrow mesenchymal stem cells for bone repair

  • 期刊论文

  • 发表日期：

    2025年3月31日

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  • Özlem Altundag-Erdogan,
  • Hayrullah Çetinkaya,
  • Betül Çelebi-Saltik,
  • Belgin Altun,
  • Mehtap Şahin,
  • Gamze Kara Mağden,
  • Mustafa Özgür Öteyaka

摘要

A triaxial nanofiber membrane was developed using a custom triaxial nozzle during electrospinning. The membrane consisted of three layers outer (PVA-ZnO), middle (PVA-Collagen/Type I-BMP-2), and inner (PVA-nHAP). TEM revealed the diameters of the outer, middle, and inner layers as 167.5, 142.6, and 119.3 nm, respectively. The triaxial nanofiber membrane exhibited superior tensile strength (36 MPa) and elastic modulus (0.025 GPa), along with a roughness of 359 nm and hydrophilic properties. In addition, it demonstrated a 200% swelling ratio and reduced bacterial colonization by 10.8-fold. Both Calcium deposition and ALP activity were significantly higher and increased expression of BSP (eightfold), OCN (13-fold), and OPN (488-fold) genes were observed in membrane + OST groups (p < 0.05), compared to MSC and OST groups. These findings highlight that the membrane has potential for enhance bone repair by combining well-structured, antimicrobial, mechanical, and osteoinductive properties.

Graphical Abstract