Scholarlink

Aim <p>In the present study, cow dung from indigenous and exotic breeds of cow were explored for its microbial population, plant growth-promoting (PGP) traits, enzyme activity, antagonistic activity, and pot experiment studies to evaluate the effect of bioinoculants under net house conditions.</p> Materials and methods <p>Physicochemical properties of cow dung, qualitative and quantitative method of PGP characterization, agar diffusion method for antifungal activity, 16Sr RNA gene sequencing method for identification, and pot experiment studies were performed.</p> Results <p>Nitrogen (N), phosphorus (P), potassium (K), electrical conductivity (EC), and pH were found maximum in indigenous <i>Himachali Pahari</i> non-lactating cow, whereas highest microbial count was found in <i>Himachali Pahari</i> lactating cow. Fourteen cow dung isolates from different breeds were found positive for all PGP, enzyme, and antifungal activities except hydrogen cyanide (HCN) production and were selected for further studies. Quantitative estimation showed isolate JD3 and JD4 isolated from Jersey non-lactating cow best in phosphate (P) solubilization (127.79&#xa0;µg&#xa0;ml<sup>−1</sup>) and siderophore production (98.42%), whereas PL2 isolated from <i>Himachali Pahari</i> lactating cow was found best in IAA production (80.03&#xa0;µg&#xa0;ml<sup>−1</sup>). Maximum antifungal activity was found in Jersey lactating and non-lactating cow dung isolates (JL1, JL2, JL4 and JD1), against all tested fungal phytopathogens. <i>Microbacterium thalassium</i> strain PL3 was first time reported from <i>Himachali Pahari</i> lactating cow. Pot experiment studies found that maximum plant height was recorded in <i>Himachali Pahari</i> lactating isolate (PL2) (14.76&#xa0;cm), germination percent was recorded highest in Jersey lactating cow dung isolates (JL4, JL1) and control (91.77%), minimum days to 1st flowering were found in Jersey isolate (JL4 and JD1) (49.33&#xa0;days) treatment, and pod number per plant was noted maximum in <i>Himachali Pahari</i> non-lactating (PD5) (3.33). Maximum chemical properties, viz., N (313.60&#xa0;kg&#xa0;ha<sup>−1</sup>), P (40.31&#xa0;kg&#xa0;ha<sup>−1</sup>), and microbial count (4.7 × 10<sup>8</sup>&#xa0;cfu&#xa0;g<sup>−1</sup>) were found in <i>Himachali Pahari</i> lactating cow isolate (PL2) treatment, while K (253.74&#xa0;kg&#xa0;ha<sup>−1</sup>) was found to be maximum in <i>Himachali Pahari</i> non-lactating cow isolate (PD3) which was statistically significant than initial values of microbial count, N, P, and K.</p> Impact statement <p>These potential five strains, i.e., PL2, PD5, JL1, JL4, and JD1, isolated from cow dung of indigenous <i>Himachali Pahari</i> and exotic Jersey breeds irrespective of lactating and non-lactating in individual or in consortium can be potential means to protect, enrich, and flourish the soil microbial community as well as plant and soil health.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Plant growth-promoting bacteria from dung of indigenous and exotic cow breeds and their effect on the growth of pea plant in sustainable agriculture

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年2月21日

查看全文
  • Shweta Sagar,
  • Arjun Singh,
  • Jyoti Bala,
  • Rakesh Chauhan,
  • Rameshwar Kumar,
  • Anila Badiyal,
  • Abhishek Walia

摘要

Aim

In the present study, cow dung from indigenous and exotic breeds of cow were explored for its microbial population, plant growth-promoting (PGP) traits, enzyme activity, antagonistic activity, and pot experiment studies to evaluate the effect of bioinoculants under net house conditions.

Materials and methods

Physicochemical properties of cow dung, qualitative and quantitative method of PGP characterization, agar diffusion method for antifungal activity, 16Sr RNA gene sequencing method for identification, and pot experiment studies were performed.

Results

Nitrogen (N), phosphorus (P), potassium (K), electrical conductivity (EC), and pH were found maximum in indigenous Himachali Pahari non-lactating cow, whereas highest microbial count was found in Himachali Pahari lactating cow. Fourteen cow dung isolates from different breeds were found positive for all PGP, enzyme, and antifungal activities except hydrogen cyanide (HCN) production and were selected for further studies. Quantitative estimation showed isolate JD3 and JD4 isolated from Jersey non-lactating cow best in phosphate (P) solubilization (127.79 µg ml−1) and siderophore production (98.42%), whereas PL2 isolated from Himachali Pahari lactating cow was found best in IAA production (80.03 µg ml−1). Maximum antifungal activity was found in Jersey lactating and non-lactating cow dung isolates (JL1, JL2, JL4 and JD1), against all tested fungal phytopathogens. Microbacterium thalassium strain PL3 was first time reported from Himachali Pahari lactating cow. Pot experiment studies found that maximum plant height was recorded in Himachali Pahari lactating isolate (PL2) (14.76 cm), germination percent was recorded highest in Jersey lactating cow dung isolates (JL4, JL1) and control (91.77%), minimum days to 1st flowering were found in Jersey isolate (JL4 and JD1) (49.33 days) treatment, and pod number per plant was noted maximum in Himachali Pahari non-lactating (PD5) (3.33). Maximum chemical properties, viz., N (313.60 kg ha−1), P (40.31 kg ha−1), and microbial count (4.7 × 108 cfu g−1) were found in Himachali Pahari lactating cow isolate (PL2) treatment, while K (253.74 kg ha−1) was found to be maximum in Himachali Pahari non-lactating cow isolate (PD3) which was statistically significant than initial values of microbial count, N, P, and K.

Impact statement

These potential five strains, i.e., PL2, PD5, JL1, JL4, and JD1, isolated from cow dung of indigenous Himachali Pahari and exotic Jersey breeds irrespective of lactating and non-lactating in individual or in consortium can be potential means to protect, enrich, and flourish the soil microbial community as well as plant and soil health.

Graphical Abstract