Scholarlink

<p>The efficient biosynthesis is important for the sustainable development of lignocellulosic ethanol industry, but it is limited by furfural stress produced with cellulose pretreatment. Collagen peptide (CP), as an affluent protein resource, considerably improved the tolerance of <i>Saccharomyces cerevisiae</i> against furfural stress. When the furfural concentration was 2&#xa0;g/L, the residual sugar concentration was reduced from 122.39 to 8.90&#xa0;g/L, and the final ethanol yield increased from 30.69 to 87.27&#xa0;g/L in the presence of CP. In addition, the ethanol yield in CP containing media was higher than those in other peptides. Transcriptome analysis showed CP can improve the expression of genes (<i>FBA1</i>, <i>PDC1</i>, <i>PDC6</i>, and <i>ENO1</i>) associated with glycolysis to promote sugar utilization, and enhance ethanol biosynthesis under furfural stress, which were further verified by quantitative real-time PCR. These results indicated that CP is a promising protectant and accelerator for bioethanol biosynthesis.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Collagen peptide protects Saccharomyces cerevisiae from furfural stress for enhancing bioethanol synthesis

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年2月2日

查看全文
  • Ming Yang,
  • Xia Li,
  • Bo Wang,
  • Xian Liu,
  • Bo Zhang,
  • Xue-Pin Liao

摘要

The efficient biosynthesis is important for the sustainable development of lignocellulosic ethanol industry, but it is limited by furfural stress produced with cellulose pretreatment. Collagen peptide (CP), as an affluent protein resource, considerably improved the tolerance of Saccharomyces cerevisiae against furfural stress. When the furfural concentration was 2 g/L, the residual sugar concentration was reduced from 122.39 to 8.90 g/L, and the final ethanol yield increased from 30.69 to 87.27 g/L in the presence of CP. In addition, the ethanol yield in CP containing media was higher than those in other peptides. Transcriptome analysis showed CP can improve the expression of genes (FBA1, PDC1, PDC6, and ENO1) associated with glycolysis to promote sugar utilization, and enhance ethanol biosynthesis under furfural stress, which were further verified by quantitative real-time PCR. These results indicated that CP is a promising protectant and accelerator for bioethanol biosynthesis.

Graphical abstract