Scholarlink

<p>Diarrhea is a major cause of mortality in Jinhua piglets, accounting for up to 80% of total piglet deaths and severely restricting the sustainability of pig production systems. In this study, colonic morphology, organ indices, gut microbiota composition, and transcriptomic profiles were compared between diarrheal piglets (DG) and healthy piglets (HG) to identify diarrhea associated bacteria in Jinhua piglets and to explore potential microbial intervention strategies. The results revealed that diarrheal piglets had lower organ indices (<i>p</i> &lt; 0.01) and worse intestinal conditions such as inflammatory factor infiltration, reduced number of goblet cells (<i>p</i> &lt; 0.05), and increased colonic crypt depth (<i>p</i> &lt; 0.05). Transcriptomics analysis of colonic mucosa revealed that differentially expressed genes (DEGs) between two groups (FC &gt; 1.5, <i>p</i> &lt; 0.05) were enriched mainly in Th1 and Th2 cell differentiation and Th17 cell differentiation. Microbiota analysis showed that bacteria such as <i>Clostridium_sensu_stricto_1</i>, which were enriched in the colon of piglets in HG, may contribute to host metabolic and immune modulation via metabolic products such as short chain fatty acids and crosstalk with other microbial taxa. In contrast, potential harmful bacteria, particularly <i>Fusobacterium,</i> were significantly enriched in the colon of piglets in DG. Correlation analysis further identified <i>IL-2Rβ</i>, <i>TBX21</i>, and <i>LCK</i> as key immune related genes associated with differentially abundant microbes. Enriched <i>Fusobacterium</i> showed associations with inflammatory gene expression, whereas <i>Clostridium_sensu_stricto_1</i> exhibited opposite correlations. In conclusion, harmful bacterial colonization may promote intestinal inflammation and diarrhea in Jinhua piglets, while beneficial bacteria exert protective effects that support piglets’ growth and intestinal health. These beneficial microbes may serve as potential probiotic candidates for improving the health of Jinhua piglets.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Disordered colonic microbiota as a potential contributor to inflammation in diarrheal Jinhua weaned piglets

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月27日

查看全文
  • Jiaheng Li,
  • Chengzeng Luo,
  • Fangfang Lou,
  • Xiaojun Zhang,
  • Jiujun Duan,
  • Zeou Wei,
  • Xujin Hu,
  • Pingguang Tu,
  • Xizhong Du,
  • Ruqing Zhong,
  • Liang Chen,
  • Hongfu Zhang

摘要

Diarrhea is a major cause of mortality in Jinhua piglets, accounting for up to 80% of total piglet deaths and severely restricting the sustainability of pig production systems. In this study, colonic morphology, organ indices, gut microbiota composition, and transcriptomic profiles were compared between diarrheal piglets (DG) and healthy piglets (HG) to identify diarrhea associated bacteria in Jinhua piglets and to explore potential microbial intervention strategies. The results revealed that diarrheal piglets had lower organ indices (p < 0.01) and worse intestinal conditions such as inflammatory factor infiltration, reduced number of goblet cells (p < 0.05), and increased colonic crypt depth (p < 0.05). Transcriptomics analysis of colonic mucosa revealed that differentially expressed genes (DEGs) between two groups (FC > 1.5, p < 0.05) were enriched mainly in Th1 and Th2 cell differentiation and Th17 cell differentiation. Microbiota analysis showed that bacteria such as Clostridium_sensu_stricto_1, which were enriched in the colon of piglets in HG, may contribute to host metabolic and immune modulation via metabolic products such as short chain fatty acids and crosstalk with other microbial taxa. In contrast, potential harmful bacteria, particularly Fusobacterium, were significantly enriched in the colon of piglets in DG. Correlation analysis further identified IL-2Rβ, TBX21, and LCK as key immune related genes associated with differentially abundant microbes. Enriched Fusobacterium showed associations with inflammatory gene expression, whereas Clostridium_sensu_stricto_1 exhibited opposite correlations. In conclusion, harmful bacterial colonization may promote intestinal inflammation and diarrhea in Jinhua piglets, while beneficial bacteria exert protective effects that support piglets’ growth and intestinal health. These beneficial microbes may serve as potential probiotic candidates for improving the health of Jinhua piglets.

Graphical Abstract