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Background <p>Cotton (<i>Gossypium</i> spp.) is an important commercial crop being cultivated worldwide, but its production is hampered by many insect pests. The cotton aphid, <i>Aphis gossypii</i> Glover, is a key pest with increasing resistance to chemical insecticides. To explore eco-friendly management alternatives, this study evaluates the pathogenic potential of indigenous entomopathogenic fungi, isolated from cotton-growing regions of Tamil Nadu, India, via the ‘<i>Galleria</i> bait method’.</p> Results <p>Five entomopathogenic fungi were isolated and identified as <i>Beauveria</i> spp. based on cultural and morphological features. Molecular characterization by amplification of internal transcribed spacer-ribosomal DNA (ITS-rDNA) regions confirmed the isolates as <i>B. bassiana</i>. Among them, isolate B5 (accession number: PP503009) exhibited the highest virulence, inducing 96.67% mortality at 7&#xa0;days after treatment (DAT) with the concentration of 1 × 10<sup>8</sup> spores·mL<sup>−1</sup>. The median lethal concentration (LC<sub>50</sub>) and median lethal time (LT<sub>50</sub>) values were 9.75 × 10<sup>4</sup> spores·mL<sup>−1</sup> at 7 DAT and 72.31&#xa0;h at 1 × 10<sup>8</sup> spores·mL<sup>−1</sup>, respectively. Scanning electron microscopy (SEM) images highlighted a progression of infection stages of B5, including spore attachment (24&#xa0;h post infection (hpi)), hyphal penetration (48 hpi), and conidiogenesis (72 hpi).</p> Conclusion <p>The isolate B5 proved to be a promising candidate for the development of biopesticides for sustainable cotton aphid management in Tamil Nadu, India.</p>

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Harnessing the potential of indigenous entomopathogenic fungi isolated from the soils of Tamil Nadu, India, for the management of the cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae)

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  • 发表日期：

    2025年9月26日

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  • Mariappan Krishnaveni,
  • Samuel Jeyarajan Nelson,
  • Uthandi Sivakumar,
  • Sevugapperumal Nakkeeran,
  • Subbarayalu Mohankumar,
  • Krishnamoorthi Premalatha

摘要

Background

Cotton (Gossypium spp.) is an important commercial crop being cultivated worldwide, but its production is hampered by many insect pests. The cotton aphid, Aphis gossypii Glover, is a key pest with increasing resistance to chemical insecticides. To explore eco-friendly management alternatives, this study evaluates the pathogenic potential of indigenous entomopathogenic fungi, isolated from cotton-growing regions of Tamil Nadu, India, via the ‘Galleria bait method’.

Results

Five entomopathogenic fungi were isolated and identified as Beauveria spp. based on cultural and morphological features. Molecular characterization by amplification of internal transcribed spacer-ribosomal DNA (ITS-rDNA) regions confirmed the isolates as B. bassiana. Among them, isolate B5 (accession number: PP503009) exhibited the highest virulence, inducing 96.67% mortality at 7 days after treatment (DAT) with the concentration of 1 × 108 spores·mL−1. The median lethal concentration (LC50) and median lethal time (LT50) values were 9.75 × 104 spores·mL−1 at 7 DAT and 72.31 h at 1 × 108 spores·mL−1, respectively. Scanning electron microscopy (SEM) images highlighted a progression of infection stages of B5, including spore attachment (24 h post infection (hpi)), hyphal penetration (48 hpi), and conidiogenesis (72 hpi).

Conclusion

The isolate B5 proved to be a promising candidate for the development of biopesticides for sustainable cotton aphid management in Tamil Nadu, India.