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<p>Dengue is a viral infection transmitted by mosquitoes of the <i>Aedes</i> genus, responsible for millions of cases each year. In Benin, several outbreaks have been reported, particularly in Abomey-Calavi, where the first dengue-related death was recorded in 2019. In the absence of a specific treatment, vector control remains the primary preventive measure. However, the emergence of insecticide resistance, notably through <i>kdr</i> mutations, could compromise its effectiveness. This study aims to identify the dengue virus serotypes circulating in the commune of Abomey-Calavi and to assess the frequency of <i>kdr</i> mutations <i>V410L</i>,<i> V1016G</i>,<i> V1016I</i>,<i> F1534C</i>,<i> and S989P</i> in <i>Aedes aegypti</i> and <i>Aedes albopictus</i> mosquitoes. Mosquitoes were collected in August 2024 from four arrondissement Togba, Calavi, Akassato, and Godomey through morning and afternoon spraying. After morphological identification, molecular analyses were conducted to detect the viral serotypes and <i>kdr</i> mutations. A total of 218 <i>Aedes</i> mosquitoes were collected, with a predominance of <i>Aedes aegypti</i> (68.8%) compared to <i>Aedes albopictus</i> (31.2%). The overall dengue positivity rate was 23.39% (95% CI: 17.9–29.6). The most frequent serotype was DENV-3 (12.4%), followed by DENV-2 (6.9%), DENV-1 (5%), and DENV-4 (0.09%). Six mosquitoes were co-infected with two serotypes. <i>Kdr</i> mutations were detected in both species, with frequencies of 45% for <i>V1016G</i>, 44% for <i>S989P</i>, 40% for <i>F1534C</i>, 22% for <i>V410L</i>, and 19% for <i>V1016I.</i> Four mosquitoes carried three simultaneous mutations, while twenty-two carried two. These results highlight the active circulation of the dengue virus and the presence of <i>kdr</i> mutations in <i>Aedes</i> mosquitoes in Abomey-Calavi. However, no significant association was observed between dengue infection and <i>kdr</i> mutations, and their distribution was independent of the viral serotype. These findings emphasize the need for regular monitoring of <i>kdr</i> mutations to adapt vector control strategies and limit the spread of dengue.</p>

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Serotypes of dengue virus and Kdr mutations (F1534C, S989P, V410L, V1016I, and V1016G) in Aedes aegypti and Aedes albopictus mosquitoes from the commune of Abomey-Calavi, Southern Benin

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  • 发表日期：

    2026年7月27日

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  • Tatchémè Filémon Tokponnon,
  • Oloubo Djibril Amoussa,
  • Zoulkifilou Sare Dabou,
  • Nicodème Kpemasse,
  • Minassou Juvenal Ahouandjinou,
  • Linda Towakinou,
  • Brunelle Agassounon,
  • Olivier Sègla Tandjekpon,
  • Fabrice Comlan Gandaho,
  • Mistourath Issa,
  • Idayath Gounou Yérima,
  • Festus Houessinon,
  • Victorien Dougnon,
  • Louisa A. Messenger,
  • Razaki Osse,
  • Lamine Baba-Moussa,
  • Dorothée Kinde Gazard,
  • Martin Akogbeto

摘要

Dengue is a viral infection transmitted by mosquitoes of the Aedes genus, responsible for millions of cases each year. In Benin, several outbreaks have been reported, particularly in Abomey-Calavi, where the first dengue-related death was recorded in 2019. In the absence of a specific treatment, vector control remains the primary preventive measure. However, the emergence of insecticide resistance, notably through kdr mutations, could compromise its effectiveness. This study aims to identify the dengue virus serotypes circulating in the commune of Abomey-Calavi and to assess the frequency of kdr mutations V410L, V1016G, V1016I, F1534C, and S989P in Aedes aegypti and Aedes albopictus mosquitoes. Mosquitoes were collected in August 2024 from four arrondissement Togba, Calavi, Akassato, and Godomey through morning and afternoon spraying. After morphological identification, molecular analyses were conducted to detect the viral serotypes and kdr mutations. A total of 218 Aedes mosquitoes were collected, with a predominance of Aedes aegypti (68.8%) compared to Aedes albopictus (31.2%). The overall dengue positivity rate was 23.39% (95% CI: 17.9–29.6). The most frequent serotype was DENV-3 (12.4%), followed by DENV-2 (6.9%), DENV-1 (5%), and DENV-4 (0.09%). Six mosquitoes were co-infected with two serotypes. Kdr mutations were detected in both species, with frequencies of 45% for V1016G, 44% for S989P, 40% for F1534C, 22% for V410L, and 19% for V1016I. Four mosquitoes carried three simultaneous mutations, while twenty-two carried two. These results highlight the active circulation of the dengue virus and the presence of kdr mutations in Aedes mosquitoes in Abomey-Calavi. However, no significant association was observed between dengue infection and kdr mutations, and their distribution was independent of the viral serotype. These findings emphasize the need for regular monitoring of kdr mutations to adapt vector control strategies and limit the spread of dengue.