Scholarlink

Background <p>Ticks and tick-borne diseases continues to be a global problem with direct and indirect impact on both humans and animals. The use of acaricides has been a common tick control method because of their ease of use and commercial availability; however, there are rising cases of resistance to synthetic acaricides, resulting in serious economic losses. This study aimed to evaluate the acaricidal activity of crude extracts and fractions from the leaves of <i>Croton hirtus</i> against the cattle tick <i>Rhipicephalus annulatus</i>. An adult immersion test method was adopted to assess the acaricidal potential of the <i>C. hirtus</i> extract and fractions at varied concentrations (1–20%), with 2% Tween-80 and 2% cypermethrin serving as negative and positive controls, respectively. Field ticks from cattle were collected, grouped (n = 10), and exposed to various test preparations. Tarsal reflexes, photosensitivity, reproductive indices, and oviposition inhibition were measured at 6&#xa0;h post-immersion and daily for 14&#xa0;days. Microsoft Excel® 2017 spreadsheet was used for data collation, and results were analysed with one-way ANOVA and Tukey HSD test on GraphPad (Prism 7), with <i>p</i> ≤ 0.05 considered statistically significant.</p> Results <p>Findings showed 100% tick mortality and a 95% inhibition of oviposition with the chloroform fraction (1%). The egg mass varied across the treatment groups, with the chloroform fraction being the least (&lt; 10&#xa0;mg), while the aqueous extract and other fractions were like the negative control. The mean adult mortality rate was not statistically different across the treatments.</p> Conclusion <p>The chloroform fraction (1%) of <i>C. hirtus</i> leaves exhibited superior acaricidal activity against <i>R. annulatus</i> ticks compared with other fractions. Future research should explore and document the mechanisms of action of this essential oil as a natural tick control remedy.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

In vitro acaricidal efficacies of crude extract and fractions of Croton hirtus leaf against Rhipicephalus annulatus

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月30日

查看全文
  • Oluwatodimu Adekoya,
  • Genesis Adewuyi,
  • Olubukola Adenubi,
  • Foluke Akande,
  • Adeoye Oyewusi

摘要

Background

Ticks and tick-borne diseases continues to be a global problem with direct and indirect impact on both humans and animals. The use of acaricides has been a common tick control method because of their ease of use and commercial availability; however, there are rising cases of resistance to synthetic acaricides, resulting in serious economic losses. This study aimed to evaluate the acaricidal activity of crude extracts and fractions from the leaves of Croton hirtus against the cattle tick Rhipicephalus annulatus. An adult immersion test method was adopted to assess the acaricidal potential of the C. hirtus extract and fractions at varied concentrations (1–20%), with 2% Tween-80 and 2% cypermethrin serving as negative and positive controls, respectively. Field ticks from cattle were collected, grouped (n = 10), and exposed to various test preparations. Tarsal reflexes, photosensitivity, reproductive indices, and oviposition inhibition were measured at 6 h post-immersion and daily for 14 days. Microsoft Excel® 2017 spreadsheet was used for data collation, and results were analysed with one-way ANOVA and Tukey HSD test on GraphPad (Prism 7), with p ≤ 0.05 considered statistically significant.

Results

Findings showed 100% tick mortality and a 95% inhibition of oviposition with the chloroform fraction (1%). The egg mass varied across the treatment groups, with the chloroform fraction being the least (< 10 mg), while the aqueous extract and other fractions were like the negative control. The mean adult mortality rate was not statistically different across the treatments.

Conclusion

The chloroform fraction (1%) of C. hirtus leaves exhibited superior acaricidal activity against R. annulatus ticks compared with other fractions. Future research should explore and document the mechanisms of action of this essential oil as a natural tick control remedy.