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<p>A crucial treatment technique for halting the onset of malnutrition in hospitalized patients is the enteral tube feeding (ETF) delivery system. The microbiological diversity of ETFs plays a significant role on patient health, especially for individuals with impaired immune systems. This study aims to assess the microbial contamination in ETF samples collected from five different hospitals, identify the dominant bacterial species, and trace the sources of nosocomial contamination using phylogenetic analysis. Following PCR amplification, 16S rRNA gene sequencing was used to characterize the isolated bacteria on a molecular level. No specimen had a total mesophilic bacteria (TMB) count of zero, according to the analysis of the ETF samples, whereas 60% (36/60) of the ETF samples included at least ≥ 10<sup>4</sup> colony-forming units (CFU) of TMB, ≥ 10<sup>3</sup>&#xa0;CFU of <i>Pseudomonas khazarica</i>, ≥ 10<sup>2</sup>&#xa0;CFU of <i>Enterobacter</i> spp., and ≥ 10&#xa0;CFU of <i>Klebsiella quasivariicola</i>. The findings emphasize the importance of implementing strict microbiological control measures at critical control points during ETF preparation to minimize cross-contamination and ensure the safety of these feeding products.</p>

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Assessment of the safety and microbial quality of enteral tube feedings via 16S rRNA-based sequencing

  • 期刊论文

  • 发表日期：

    2025年3月3日

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  • Amin Abbasi,
  • Masoud Hassanvand Amouzadeh,
  • Samaneh Salehipour Bavarsad,
  • Sahar Sabahi,
  • Zahra Farshadzadeh,
  • Behroz Taheri

摘要

A crucial treatment technique for halting the onset of malnutrition in hospitalized patients is the enteral tube feeding (ETF) delivery system. The microbiological diversity of ETFs plays a significant role on patient health, especially for individuals with impaired immune systems. This study aims to assess the microbial contamination in ETF samples collected from five different hospitals, identify the dominant bacterial species, and trace the sources of nosocomial contamination using phylogenetic analysis. Following PCR amplification, 16S rRNA gene sequencing was used to characterize the isolated bacteria on a molecular level. No specimen had a total mesophilic bacteria (TMB) count of zero, according to the analysis of the ETF samples, whereas 60% (36/60) of the ETF samples included at least ≥ 104 colony-forming units (CFU) of TMB, ≥ 103 CFU of Pseudomonas khazarica, ≥ 102 CFU of Enterobacter spp., and ≥ 10 CFU of Klebsiella quasivariicola. The findings emphasize the importance of implementing strict microbiological control measures at critical control points during ETF preparation to minimize cross-contamination and ensure the safety of these feeding products.