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<p>Two different hydrolysates of the water-soluble protein (WSP) fraction from tuna fish meal were obtained by subcritical water-CO<sub>2</sub> (subW-CO<sub>2</sub>) and Alcalase<sup>®</sup> treatments. Hydrolysates showed different chemical composition regarding their free amino acid (FAA) profile and molecular weight distribution of the peptides generated. Consequently, different strategies were proposed by using a stirred dead-end filtration system equipped with flat sheet membranes. Two nanofiltration (NF) consecutive steps were proposed for fractionation of subW-CO<sub>2</sub> hydrolysates with polyamide membranes (800–600&#xa0;Da and 300–150&#xa0;Da) yielding a first retentate where 79% of the protein fraction was retained, while 92% of FAA were retained in the second step with a purity index of 29%. Alcalase<sup>®</sup> hydrolysates were fractionated by ultrafiltration (UF, 10&#xa0;kDa) followed by NF (1–1.1&#xa0;kDa) with polyethersulfone membranes obtaining a first retentate with more than 65% of protein fraction retention with a purity index of 50%. A&#xa0;relatively wide molecular weight distribution was observed in all streams, with&#xa0;high antioxidant and iron-chelating capacities&#xa0;in the retentate&#xa0;fractions.</p> Graphical Abstract <p></p>

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Membrane fractionation of hydrolysates of the water-soluble protein from tuna fish meal obtained by subcritical water and enzymatic treatments. Comparison of physical and chemical properties

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  • 发表日期：

    2025年3月6日

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  • Pedro Barea,
  • Alba Ester Illera,
  • Helena Candela,
  • Rodrigo Melgosa,
  • José Manuel Benito,
  • Sagrario Beltrán,
  • María Teresa Sanz

摘要

Two different hydrolysates of the water-soluble protein (WSP) fraction from tuna fish meal were obtained by subcritical water-CO2 (subW-CO2) and Alcalase® treatments. Hydrolysates showed different chemical composition regarding their free amino acid (FAA) profile and molecular weight distribution of the peptides generated. Consequently, different strategies were proposed by using a stirred dead-end filtration system equipped with flat sheet membranes. Two nanofiltration (NF) consecutive steps were proposed for fractionation of subW-CO2 hydrolysates with polyamide membranes (800–600 Da and 300–150 Da) yielding a first retentate where 79% of the protein fraction was retained, while 92% of FAA were retained in the second step with a purity index of 29%. Alcalase® hydrolysates were fractionated by ultrafiltration (UF, 10 kDa) followed by NF (1–1.1 kDa) with polyethersulfone membranes obtaining a first retentate with more than 65% of protein fraction retention with a purity index of 50%. A relatively wide molecular weight distribution was observed in all streams, with high antioxidant and iron-chelating capacities in the retentate fractions.

Graphical Abstract