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<p>Improper disposal of jackfruit peel waste poses environmental risks, but its polysaccharides (PJP) offer potential health benefits. This study aimed to optimize the ultrasound-microwave-assisted extraction conditions for PJP and evaluate their structural characteristics as well as intestinal health activity. Using Box–Behnken design, the optimal extraction yield of 24.6% was achieved under the following conditions: solid-to-liquid ratio of 48.7&#xa0;mL/g, extraction time of 29.8&#xa0;min, and extraction temperature of 81.9&#xa0;°C. Structural analysis revealed that PJP primarily consists of glucose units with a backbone linked by → 4)-β-D-Glc<i>p</i>-(1 →. In DSS-induced colitis mice, PJP increased the abundance of intestinal microorganisms (<i>Bacteroides</i> and <i>Lactobacillus</i>), enhanced the production of short-chain fatty acids (SCFAs), and reduced inflammation (TNF-α, IL-1β), while enhancing the levels of tight junction proteins (claudin-1, occludin, ZO-1). This study provides a methodological framework for polysaccharide research and provides preliminary evidence for the industrial production and practical application of PJP. </p> Graphical Abstract <p></p>

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Optimizing ultrasound-microwave-assisted extraction of jackfruit peel polysaccharides and evaluating their impact on intestinal health in vivo

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月22日

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  • Kit-Leong Cheong,
  • Keying Liu,
  • Yun-Feng Li,
  • Chen Huang,
  • Saiyi Zhong,
  • Karsoon Tan,
  • Malairaj Sathuvan

摘要

Improper disposal of jackfruit peel waste poses environmental risks, but its polysaccharides (PJP) offer potential health benefits. This study aimed to optimize the ultrasound-microwave-assisted extraction conditions for PJP and evaluate their structural characteristics as well as intestinal health activity. Using Box–Behnken design, the optimal extraction yield of 24.6% was achieved under the following conditions: solid-to-liquid ratio of 48.7 mL/g, extraction time of 29.8 min, and extraction temperature of 81.9 °C. Structural analysis revealed that PJP primarily consists of glucose units with a backbone linked by → 4)-β-D-Glcp-(1 →. In DSS-induced colitis mice, PJP increased the abundance of intestinal microorganisms (Bacteroides and Lactobacillus), enhanced the production of short-chain fatty acids (SCFAs), and reduced inflammation (TNF-α, IL-1β), while enhancing the levels of tight junction proteins (claudin-1, occludin, ZO-1). This study provides a methodological framework for polysaccharide research and provides preliminary evidence for the industrial production and practical application of PJP.

Graphical Abstract