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<p>Frontotemporal dementia linked to chromosome 3 (FTD3) is caused by a splice site point mutation in <i>CHMP2B</i>, resulting in the production of mutant proteins CHMP2B<sup>In5</sup> and CHMP2B<sup>Δ10</sup>. Here, we found that wildtype CHMP2B (CHMP2B<sup>WT</sup>) is mostly present in the cytoplasm, but CHMP2B<sup>In5</sup> is mislocalized to the nucleus of human induced pluripotent stem cell (iPSC)-derived cortical neurons. To understand the underlying mechanism, we identified a previously unreported nuclear export signal (NES) in the C-terminus of CHMP2B. Functional assays, including CRM1 inhibition and site-directed mutagenesis of key hydrophobic residues, demonstrated that this NES motif is both necessary and sufficient for nuclear export of CHMP2B<sup>WT</sup> and ALS-associated CHMP2B<sup>Q206H</sup>, and its loss in CHMP2B<sup>In5</sup> is responsible for the observed nuclear mislocalization. CHMP2B<sup>Δ10</sup> remains in the cytoplasm due to the presence of an artificial NES in the C-terminus. These results reveal the presence of an NES in CHMP2B and highlight the need to dissect the gain-of-toxic nuclear functions of CHMP2B<sup>In5</sup> in FTD3 pathogenesis.</p>

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Mislocalization of FTD3-associated mutant CHMP2B to the nucleus of human neurons due to loss of a nuclear export signal

  • 期刊论文

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  • 发表日期：

    2026年1月20日

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  • Yong-Woo Jun,
  • Evan P. Hass,
  • Soojin Lee,
  • Thomas G. Fazzio,
  • Fen-Biao Gao

摘要

Frontotemporal dementia linked to chromosome 3 (FTD3) is caused by a splice site point mutation in CHMP2B, resulting in the production of mutant proteins CHMP2BIn5 and CHMP2BΔ10. Here, we found that wildtype CHMP2B (CHMP2BWT) is mostly present in the cytoplasm, but CHMP2BIn5 is mislocalized to the nucleus of human induced pluripotent stem cell (iPSC)-derived cortical neurons. To understand the underlying mechanism, we identified a previously unreported nuclear export signal (NES) in the C-terminus of CHMP2B. Functional assays, including CRM1 inhibition and site-directed mutagenesis of key hydrophobic residues, demonstrated that this NES motif is both necessary and sufficient for nuclear export of CHMP2BWT and ALS-associated CHMP2BQ206H, and its loss in CHMP2BIn5 is responsible for the observed nuclear mislocalization. CHMP2BΔ10 remains in the cytoplasm due to the presence of an artificial NES in the C-terminus. These results reveal the presence of an NES in CHMP2B and highlight the need to dissect the gain-of-toxic nuclear functions of CHMP2BIn5 in FTD3 pathogenesis.