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Background <p>The deposition of intramuscular fat (IMF) in livestock can enhance the flavor and tenderness of meat products, significantly increasing consumer satisfaction. To achieve this industrial trait, this study investigated the regulatory effects of 20 dietary nutrients on sheep IMF deposition using a 3D organoid culture model.</p> Results <p>Key nutrients enhancing angiogenesis, adipocyte differentiation, and lipid accumulation were identified through assessments of capillary sprouts development, mRNA expression, and Oil Red O staining. Vitamins C (VC), E (VE), and K<sub>1</sub> (VK1), guanidinoacetic acid (GAA), leucine (Leu), lysine (Lys), methionine (Met), N-carbamylglutamate (NCG), tryptophan (Trp), α-linolenic acid (ALA), linoleic acid (LA), <i>cis</i>-9, <i>trans</i>-11 conjugated linoleic acid (<i>c</i>9,&#xa0;<i>t</i>11-CLA), acetic acid (HAc), and sodium acetate (NaAc) stimulated while vitamins B<sub>9</sub> (VB9), D (VD), K<sub>2</sub> (VK2), taurine (Tau), and sodium butyrate (NaBu) inhibited angiogenesis (<i>P</i> &lt; 0.05). Furthermore, VC, VE, VK1, VK2, GAA, Leu, NCG, Trp, ALA, LA, and HAc enhanced adipocyte differentiation, with VE, VK1, GAA, Leu, LA, and HAc additionally elevating lipid accumulation (<i>P</i> &lt; 0.05).</p> Conclusions <p>Various nutrients play distinct regulatory roles in angiogenesis, adipocyte differentiation, and lipid accumulation. These findings provide a roadmap for further optimizing the production of marbled meat through nutritional intervention in actual livestock breeding production.&#xa0;</p> Graphical Abstract <p></p>

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In vitro screening of nutrients regulating sheep intramuscular angiogenesis, adipogenesis, and lipid deposition using an organoid model

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月27日

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  • Yating Li,
  • Xiaoying Sun,
  • Yue Lv,
  • Jiaxin Liu,
  • Xinyi Mao,
  • Jinyan Yu,
  • Yanrong Feng,
  • Long Cheng,
  • Yifan Xiang,
  • Yu Xin,
  • Zhongzuo Huang,
  • Yichen Luo,
  • Yan Zhang,
  • Junxing Zhao,
  • Bo Wang

摘要

Background

The deposition of intramuscular fat (IMF) in livestock can enhance the flavor and tenderness of meat products, significantly increasing consumer satisfaction. To achieve this industrial trait, this study investigated the regulatory effects of 20 dietary nutrients on sheep IMF deposition using a 3D organoid culture model.

Results

Key nutrients enhancing angiogenesis, adipocyte differentiation, and lipid accumulation were identified through assessments of capillary sprouts development, mRNA expression, and Oil Red O staining. Vitamins C (VC), E (VE), and K1 (VK1), guanidinoacetic acid (GAA), leucine (Leu), lysine (Lys), methionine (Met), N-carbamylglutamate (NCG), tryptophan (Trp), α-linolenic acid (ALA), linoleic acid (LA), cis-9, trans-11 conjugated linoleic acid (c9, t11-CLA), acetic acid (HAc), and sodium acetate (NaAc) stimulated while vitamins B9 (VB9), D (VD), K2 (VK2), taurine (Tau), and sodium butyrate (NaBu) inhibited angiogenesis (P < 0.05). Furthermore, VC, VE, VK1, VK2, GAA, Leu, NCG, Trp, ALA, LA, and HAc enhanced adipocyte differentiation, with VE, VK1, GAA, Leu, LA, and HAc additionally elevating lipid accumulation (P < 0.05).

Conclusions

Various nutrients play distinct regulatory roles in angiogenesis, adipocyte differentiation, and lipid accumulation. These findings provide a roadmap for further optimizing the production of marbled meat through nutritional intervention in actual livestock breeding production. 

Graphical Abstract