Scholarlink

Background and objective <p>Obesity is a significant risk factor for Central Precocious Puberty (CPP), and dietary phytoestrogens are also implicated. This study aimed to identify differential serum metabolites in girls with CPP.</p> Methods <p>This case–control study included 100 girls with CPP (CC group) and 100 healthy control girls (NN group). Serum samples were analyzed using a non-targeted metabolomics approach via UHPLC-MS/MS. Differential metabolites were identified using multivariate statistical analysis (OPLS-DA) and two-tailed Student’s <i>t</i>-test. The Comparative Toxicogenomics Database (CTD) was queried using the identified key differential metabolite to retrieve potential target genes, which were subsequently analyzed in the STRING database to predict disease associations and protein–protein interaction (PPI) networks.</p> Results <p>Principal Component Analysis (PCA) revealed distinct metabolic profile differences between CPP and control groups, with high system stability confirmed by quality control analysis (Pearson <i>r</i> &gt; 0.99). A total of 551 differential metabolites were identified, and Secoisolariciresinol was significantly elevated in all CPP subgroups (CPP with normal weight (C), overweight (COW), or obesity (COB)) compared to their non-CPP counterparts (N,NOW,NOB) (<i>P</i> &lt; 0.0001), positively correlating with BMI.Bioinformatic analysis predicted a strong link between Secoisolariciresinol and obesity and identified key protein targets including EGFR, ESR1, and IGF1R. Molecular docking subsequently confirmed a stable, high-affinity binding between Secoisolariciresinol and the active site of EGFR.</p> Conclusion <p>Secoisolariciresinol is a promising CPP biomarker, potentially involved in pathogenesis via EGFR signaling, providing new insights for diagnosis and intervention.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Secoisolariciresinol levels in Central Precocious Puberty: a metabolomic study on disease association and obesity

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月8日

查看全文
  • Haidan Li,
  • Manfang Xie,
  • Hailing Luo,
  • Yuhua Cai,
  • Li Liu,
  • Hongai Li,
  • Jing Xue,
  • Xiaojie He,
  • Xiaoyan Huang

摘要

Background and objective

Obesity is a significant risk factor for Central Precocious Puberty (CPP), and dietary phytoestrogens are also implicated. This study aimed to identify differential serum metabolites in girls with CPP.

Methods

This case–control study included 100 girls with CPP (CC group) and 100 healthy control girls (NN group). Serum samples were analyzed using a non-targeted metabolomics approach via UHPLC-MS/MS. Differential metabolites were identified using multivariate statistical analysis (OPLS-DA) and two-tailed Student’s t-test. The Comparative Toxicogenomics Database (CTD) was queried using the identified key differential metabolite to retrieve potential target genes, which were subsequently analyzed in the STRING database to predict disease associations and protein–protein interaction (PPI) networks.

Results

Principal Component Analysis (PCA) revealed distinct metabolic profile differences between CPP and control groups, with high system stability confirmed by quality control analysis (Pearson r > 0.99). A total of 551 differential metabolites were identified, and Secoisolariciresinol was significantly elevated in all CPP subgroups (CPP with normal weight (C), overweight (COW), or obesity (COB)) compared to their non-CPP counterparts (N,NOW,NOB) (P < 0.0001), positively correlating with BMI.Bioinformatic analysis predicted a strong link between Secoisolariciresinol and obesity and identified key protein targets including EGFR, ESR1, and IGF1R. Molecular docking subsequently confirmed a stable, high-affinity binding between Secoisolariciresinol and the active site of EGFR.

Conclusion

Secoisolariciresinol is a promising CPP biomarker, potentially involved in pathogenesis via EGFR signaling, providing new insights for diagnosis and intervention.