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Objective <p>To investigate miR-100-5p expression in GDM patients, assess its early predictive value for GDM development and association with glucose metabolism indicators/pregnancy outcomes, and clarify its regulatory role in trophoblast cell function.</p> Methods <p>A total of 123 patients with GDM and 120 healthy pregnant women were enrolled. RT-qPCR detected serum miR-100-5p expression. ROC curve analyzed its diagnostic value. Pearson correlation analyzed the relationship between miR-100-5p and clinical indicators. Multivariate logistic regression identified risk factors for adverse pregnancy outcomes. In vitro, HTR8/SVneo cells were treated with high glucose, and miR-100-5p inhibitor was transfected to detect cell function via CCK-8 and Transwell assays.</p> Results <p>Serum miR-100-5p in GDM group was significantly higher than control, with AUC = 0.914 for GDM diagnosis. miR-100-5p was positively correlated with HbA1c, HOMA-IR, LEP, and FBG. High miR-100-5p expression was an independent risk factor for adverse outcomes. High glucose upregulated miR-100-5p in trophoblast cells, and inhibitor reversed high glucose-induced inhibition of cell proliferation, migration and invasion.</p> Conclusion <p>miR-100-5p is highly expressed in GDM, showing promising diagnostic potential. Preliminary evidence suggests it is associated with glucose metabolism and adverse outcomes, and may serve as a potential therapeutic target, providing a basis for further investigation into GDM intervention.</p>

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Clinical significance of miR-100-5p in gestational diabetes mellitus and its impact on trophoblast cell function

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月26日

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  • Shaomeng Sun,
  • Huimin Cui,
  • Hui Jiang,
  • Mengzhu Ran,
  • Mingxiu Cui

摘要

Objective

To investigate miR-100-5p expression in GDM patients, assess its early predictive value for GDM development and association with glucose metabolism indicators/pregnancy outcomes, and clarify its regulatory role in trophoblast cell function.

Methods

A total of 123 patients with GDM and 120 healthy pregnant women were enrolled. RT-qPCR detected serum miR-100-5p expression. ROC curve analyzed its diagnostic value. Pearson correlation analyzed the relationship between miR-100-5p and clinical indicators. Multivariate logistic regression identified risk factors for adverse pregnancy outcomes. In vitro, HTR8/SVneo cells were treated with high glucose, and miR-100-5p inhibitor was transfected to detect cell function via CCK-8 and Transwell assays.

Results

Serum miR-100-5p in GDM group was significantly higher than control, with AUC = 0.914 for GDM diagnosis. miR-100-5p was positively correlated with HbA1c, HOMA-IR, LEP, and FBG. High miR-100-5p expression was an independent risk factor for adverse outcomes. High glucose upregulated miR-100-5p in trophoblast cells, and inhibitor reversed high glucose-induced inhibition of cell proliferation, migration and invasion.

Conclusion

miR-100-5p is highly expressed in GDM, showing promising diagnostic potential. Preliminary evidence suggests it is associated with glucose metabolism and adverse outcomes, and may serve as a potential therapeutic target, providing a basis for further investigation into GDM intervention.