Scholarlink

Background <p>Human immunodeficiency virus type 1 (HIV-1) causes immune deficiency, particularly CD4+T-cell depletion. This cross-sectional study explored circulating cell-free DNA (ccfDNA) as a biomarker for immune function and disease progression in HIV-1-infected individuals.</p> Methods <p>We measured ccfDNA levels, CD4+T-cell counts, immune activation/apoptosis markers, and viral load in 79 patients receiving antiretroviral therapy (37 immune responders, 42 non-responders) and 35 controls. Correlation and receiver operating characteristic (ROC) curve analysis were performed to assess relationships between ccfDNA and immunological/virological parameters.</p> Results <p>ccfDNA negatively correlated with CD4+T-cell counts (<i>r</i> = − 0.78, <i>P</i> &lt; 0.01) and positively correlated with viral load (<i>r</i> = 0.58, <i>P</i> &lt; 0.01) and T-cell immune activation/apoptosis markers (<i>r</i> = 0.78 ~ 0.89, <i>P</i> &lt; 0.01). Multivariate regression showed CD4+T-cell counts independently associated with lower ccfDNA, while CD4+AnnexinV+, CD8+PD1+, and CD8+AnnexinV+T cells correlated with higher ccfDNA. ROC analysis (area under curve = 0.799) indicated ccfDNA’s potential as a complementary biomarker for monitoring HIV-1 patients.</p> Conclusions <p>Preliminary evidence from this study indicates that ccfDNA levels are closely linked to immune dysfunction and may have potential as a complementary biomarker for monitoring immune status in HIV-1-infected patients.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Circulating cell-free DNA as a biomarker for immune function and disease progression in HIV-infected patients: a cross-sectional observational study

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月2日

查看全文
  • Lu Cheng,
  • Haokun Gao,
  • Meixin Fang,
  • Changzhong Jin

摘要

Background

Human immunodeficiency virus type 1 (HIV-1) causes immune deficiency, particularly CD4+T-cell depletion. This cross-sectional study explored circulating cell-free DNA (ccfDNA) as a biomarker for immune function and disease progression in HIV-1-infected individuals.

Methods

We measured ccfDNA levels, CD4+T-cell counts, immune activation/apoptosis markers, and viral load in 79 patients receiving antiretroviral therapy (37 immune responders, 42 non-responders) and 35 controls. Correlation and receiver operating characteristic (ROC) curve analysis were performed to assess relationships between ccfDNA and immunological/virological parameters.

Results

ccfDNA negatively correlated with CD4+T-cell counts (r = − 0.78, P < 0.01) and positively correlated with viral load (r = 0.58, P < 0.01) and T-cell immune activation/apoptosis markers (r = 0.78 ~ 0.89, P < 0.01). Multivariate regression showed CD4+T-cell counts independently associated with lower ccfDNA, while CD4+AnnexinV+, CD8+PD1+, and CD8+AnnexinV+T cells correlated with higher ccfDNA. ROC analysis (area under curve = 0.799) indicated ccfDNA’s potential as a complementary biomarker for monitoring HIV-1 patients.

Conclusions

Preliminary evidence from this study indicates that ccfDNA levels are closely linked to immune dysfunction and may have potential as a complementary biomarker for monitoring immune status in HIV-1-infected patients.