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Purpose <p>Perioperative respiratory adverse events (PRAEs) are frequently encountered complications in pediatric anesthesia. The purpose of this experiment is to explore the significant role of miR-18a-5p in PRAEs, thereby providing a novel biomarker for PRAE clinical management.</p> Methods <p>Clinical data from 174 children undergoing general anesthesia were analyzed. MiR-18a-5p expression in serum and cells was analyzed via real-time quantitative PCR (qRT-PCR). Its diagnostic value for PRAEs was evaluated through receiver operating characteristic (ROC) curves, while logistic regression was selected to assess independent risk factors for PRAEs. In vitro experiments were conducted to determine miR-18a-5p effects on AEC-II cells proliferation, apoptosis, and lipopolysaccharide (LPS)-induced tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) secretion.</p> Results <p>PRAEs subjects exhibited significantly elevated miR-18a-5p levels with diagnostic relevance. Expression correlated positively with TNF-α/IL-6 concentrations and was associated with age, obesity, American Society of Anesthesiologists (ASA) grade, and anesthesia duration. These parameters jointly constituted the PRAEs predictors. Furthermore, miR-18a-5p silencing enhanced AEC-II cells’ viability while suppressing apoptosis and LPS-triggered TNF-α and IL-6 release.</p> Conclusion <p>The miR-18a-5p served as a predictive factor and held diagnostic significance for PRAEs following general anesthesia in children. Additionally, miR-18a-5p may offer potential therapeutic implications for PRAEs.</p>

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MiR-18a-5p serves as a risk factor in perioperative respiratory adverse events in children under general anesthesia

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月28日

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  • Lin Zhang,
  • Xinyuan Yu,
  • Jun Liu,
  • Xiaotong Han,
  • Xiaoning Ma,
  • Min Li,
  • Cuiling Liu

摘要

Purpose

Perioperative respiratory adverse events (PRAEs) are frequently encountered complications in pediatric anesthesia. The purpose of this experiment is to explore the significant role of miR-18a-5p in PRAEs, thereby providing a novel biomarker for PRAE clinical management.

Methods

Clinical data from 174 children undergoing general anesthesia were analyzed. MiR-18a-5p expression in serum and cells was analyzed via real-time quantitative PCR (qRT-PCR). Its diagnostic value for PRAEs was evaluated through receiver operating characteristic (ROC) curves, while logistic regression was selected to assess independent risk factors for PRAEs. In vitro experiments were conducted to determine miR-18a-5p effects on AEC-II cells proliferation, apoptosis, and lipopolysaccharide (LPS)-induced tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) secretion.

Results

PRAEs subjects exhibited significantly elevated miR-18a-5p levels with diagnostic relevance. Expression correlated positively with TNF-α/IL-6 concentrations and was associated with age, obesity, American Society of Anesthesiologists (ASA) grade, and anesthesia duration. These parameters jointly constituted the PRAEs predictors. Furthermore, miR-18a-5p silencing enhanced AEC-II cells’ viability while suppressing apoptosis and LPS-triggered TNF-α and IL-6 release.

Conclusion

The miR-18a-5p served as a predictive factor and held diagnostic significance for PRAEs following general anesthesia in children. Additionally, miR-18a-5p may offer potential therapeutic implications for PRAEs.