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Background <p>Understanding how agricultural management practices regulate plant–soil–microbe interactions through carbon (C)–nitrogen (N)–phosphorus (P) stoichiometry is essential for optimizing nutrient cycling in orchard ecosystems. The integration of cover crops with fertilization represents a promising strategy to enhance ecosystem functionality, yet the stoichiometric pathways through which these practices jointly influence citrus productivity remain poorly quantified.</p> Method <p>A field experiment was conducted in a citrus orchard to evaluate the combined effects of fertilization and smooth vetch (<i>Vicia villosa</i> Roth var. <i>glabrescens</i> Koch) cover on soil–plant–microbial nutrient dynamics. Four treatments were established in a factorial design: VF (fertilization with smooth vetch cover), VnoF (fertilization without smooth vetch cover), VFno (no fertilization with smooth vetch cover), and VnoFno (no fertilization and no smooth vetch cover). Soil properties, leaf nutrient concentrations, microbial characteristics, and fruit yield were systematically analyzed.</p> Results <p>Smooth vetch and fertilization exhibited distinct regulatory effects on soil–plant nutrient dynamics. Smooth vetch alone exerted minimal influence on soil properties, whereas fertilization consistently reduced soil C:N ratio regardless of vetch presence, likely attributable to accelerated residue decomposition and enhanced nutrient cycling. The VF treatment significantly decreased soil C:N ratio while showing no significant effects on C:P and N:P ratios, indicating selective impacts on N rather than P dynamics. Notably, these soil responses did not directly translate to plant nutrient patterns. Leaf P concentrations varied significantly among treatments, peaking in VFno and reaching a minimum in VnoF, which drove substantial shifts in leaf C:P and N:P ratios—demonstrating a decoupling between soil and plant stoichiometric responses. Based on Random Forest analysis, soil microbial properties were the strongest predictors of leaf nutrient variations. PLS-PM analysis revealed that soil clay content had the largest total effect on yield, likely through its association with aggregate stability and nutrient retention. The VF treatment produced a mean fruit yield of 3.58 ± 0.76&#xa0;kg&#xa0;tree<sup>−1</sup>, which was 13-fold higher than VnoFno (0.28 ± 0.04&#xa0;kg&#xa0;tree<sup>−1</sup>; <i>p</i> &lt; 0.01). The entropy-weighted TOPSIS comprehensive evaluation integrating yield, soil quality, and environmental indicators confirmed VF as the optimal treatment (relative closeness coefficient <i>C</i><sub>i</sub> = 0.535 vs. 0.457 for VnoFno).</p> Conclusions <p>Integrating balanced fertilization with smooth vetch cover substantially enhances citrus orchard productivity, with microbial biomass stoichiometry identified as the strongest predictor linking soil nutrient status to leaf nutrition and yield. This combined management practice is recommended for subtropical orchards to improve nutrient cycling efficiency and ecosystem functionality, though causal mechanistic pathways require further experimental validation.</p>

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Combined smooth vetch cover and fertilization optimizes plant–soil–microbial C:N:P stoichiometry and leverages clay-associated nutrient retention to enhance citrus yield

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月14日

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  • Hongbing Yang,
  • Wenfa Xiao,
  • Lixiong Zeng,
  • Xin Yang,
  • Jiajia Zhang,
  • Zizhao Li,
  • Zhilin Huang,
  • Lei Lei

摘要

Background

Understanding how agricultural management practices regulate plant–soil–microbe interactions through carbon (C)–nitrogen (N)–phosphorus (P) stoichiometry is essential for optimizing nutrient cycling in orchard ecosystems. The integration of cover crops with fertilization represents a promising strategy to enhance ecosystem functionality, yet the stoichiometric pathways through which these practices jointly influence citrus productivity remain poorly quantified.

Method

A field experiment was conducted in a citrus orchard to evaluate the combined effects of fertilization and smooth vetch (Vicia villosa Roth var. glabrescens Koch) cover on soil–plant–microbial nutrient dynamics. Four treatments were established in a factorial design: VF (fertilization with smooth vetch cover), VnoF (fertilization without smooth vetch cover), VFno (no fertilization with smooth vetch cover), and VnoFno (no fertilization and no smooth vetch cover). Soil properties, leaf nutrient concentrations, microbial characteristics, and fruit yield were systematically analyzed.

Results

Smooth vetch and fertilization exhibited distinct regulatory effects on soil–plant nutrient dynamics. Smooth vetch alone exerted minimal influence on soil properties, whereas fertilization consistently reduced soil C:N ratio regardless of vetch presence, likely attributable to accelerated residue decomposition and enhanced nutrient cycling. The VF treatment significantly decreased soil C:N ratio while showing no significant effects on C:P and N:P ratios, indicating selective impacts on N rather than P dynamics. Notably, these soil responses did not directly translate to plant nutrient patterns. Leaf P concentrations varied significantly among treatments, peaking in VFno and reaching a minimum in VnoF, which drove substantial shifts in leaf C:P and N:P ratios—demonstrating a decoupling between soil and plant stoichiometric responses. Based on Random Forest analysis, soil microbial properties were the strongest predictors of leaf nutrient variations. PLS-PM analysis revealed that soil clay content had the largest total effect on yield, likely through its association with aggregate stability and nutrient retention. The VF treatment produced a mean fruit yield of 3.58 ± 0.76 kg tree−1, which was 13-fold higher than VnoFno (0.28 ± 0.04 kg tree−1; p < 0.01). The entropy-weighted TOPSIS comprehensive evaluation integrating yield, soil quality, and environmental indicators confirmed VF as the optimal treatment (relative closeness coefficient Ci = 0.535 vs. 0.457 for VnoFno).

Conclusions

Integrating balanced fertilization with smooth vetch cover substantially enhances citrus orchard productivity, with microbial biomass stoichiometry identified as the strongest predictor linking soil nutrient status to leaf nutrition and yield. This combined management practice is recommended for subtropical orchards to improve nutrient cycling efficiency and ecosystem functionality, though causal mechanistic pathways require further experimental validation.