Scholarlink

<p>Regulatory B cells (Bregs) are immune cells that affect the autoimmune response by producing interleukin (IL)-10, but the molecular mechanism affecting Breg differentiation and IL-10 production is not clear. Our study found that mice with limb bud and limb-bud and heart (LBH) gene deficiency in dextran sulfate sodium (DSS)-induced colitis showed severe colitis but exhibited an increase in Bregs in mesenteric lymph nodes and the spleen. LBH deficiency increased IL-10 production and expression of the surface molecule CD24 in B cells. Mechanistically, Ras-proximate-1 (Rap1) signaling pathway activation in LBH-deleted B cells and simultaneous inhibition of Rap 1 activation or extracellular signal-related kinase (ERK) phosphorylation reduced Breg differentiation among B cells. Furthermore, LBH deficient B cells showed a more significant therapeutic effect on DSS-induced colitis. Taken together, the results of this study revealed the mechanism by which LBH affects IL-10 production during Breg differentiation and that LBH deficiency enhanced the therapeutic efficacy of B cells for DSS-induced colitis.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

LBH deficiency influences the therapeutic effects of regulatory B cells through the regulation of IL-10 production via the Rap1 pathway

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月24日

查看全文
  • Weijia Bao,
  • Ke Hu,
  • Yuan Qu,
  • Yihong He,
  • Xiaotong Zhu,
  • Yuping Zhang,
  • Yage Tian,
  • Ligang Jie,
  • Qinghong Yu

摘要

Regulatory B cells (Bregs) are immune cells that affect the autoimmune response by producing interleukin (IL)-10, but the molecular mechanism affecting Breg differentiation and IL-10 production is not clear. Our study found that mice with limb bud and limb-bud and heart (LBH) gene deficiency in dextran sulfate sodium (DSS)-induced colitis showed severe colitis but exhibited an increase in Bregs in mesenteric lymph nodes and the spleen. LBH deficiency increased IL-10 production and expression of the surface molecule CD24 in B cells. Mechanistically, Ras-proximate-1 (Rap1) signaling pathway activation in LBH-deleted B cells and simultaneous inhibition of Rap 1 activation or extracellular signal-related kinase (ERK) phosphorylation reduced Breg differentiation among B cells. Furthermore, LBH deficient B cells showed a more significant therapeutic effect on DSS-induced colitis. Taken together, the results of this study revealed the mechanism by which LBH affects IL-10 production during Breg differentiation and that LBH deficiency enhanced the therapeutic efficacy of B cells for DSS-induced colitis.