Scholarlink

<p>Isolation of <i>Pythium</i> and <i>Globisporangium</i> (Chromista, Oomycota) from their natural sources is very difficult due to the heavy contamination with other microbes, which hinders obtaining them in pure form. This study aims to develop a new, cheap and safe selective medium to isolate <i>Pythium</i> and <i>Globisporangium</i> species from their natural sources, despite the previous presence of more than 3 types of selective media including: VP<sub>3</sub> (Vancomycin + PCNB + Penicillin + Pimaricin), NARM (Nystatin + Ampicillin + Rifampicin + Miconazole), and NARF (Nystatin + Ampicillin + Rifampicin + Fluazinam) used to isolate Oomycota. The elevated expense of antibiotics, along with alerts regarding potential contamination, toxicity, and tumor proliferation, renders them undesirable. Safe and inexpensive antibiotics from pharmaceutical sources were used with fixed formulations and concentrations. A selective medium was developed, called FANS (Fluconazole + Ampicillin + Nystatin + Sulbactam) with the following concentrations (1200&#xa0;mg + 500&#xa0;mg + 100,000 IU + 250&#xa0;mg) respectively/L Corn Meal Agar promotes only the growth and formation of reproductive structures of <i>Pythium</i> and <i>Globisporangium</i>. On the other hand, these antibiotics completely inhibited the growth of other contaminated fungi and bacteria. Mycelial growth, zoospore formation, and oospore production for (<i>Pythium aphanidermatum</i>,<i> Pythium oligandrum</i>, and <i>Globisporangium ultimum</i> var. <i>ultimum</i>) were highest in FANS, followed by NARM, then NARF, while VP<sub>3</sub> gave the lowest results compared to the control sample. It can be concluded that the use of FANS selective medium made the process of <i>Pythium</i> isolation easier, safer, and less expensive for worldwide researchers especially in developing and low-income countries.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A novel, cheap and easy preparing selective medium for isolation of Pythium species

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年10月13日

查看全文
  • Sahar A. Moharam,
  • Amna M. Sadek,
  • Hani M. A. Abdelzaher,
  • Mahmoud A. Shoulkamy,
  • Hussam H. Arafat

摘要

Isolation of Pythium and Globisporangium (Chromista, Oomycota) from their natural sources is very difficult due to the heavy contamination with other microbes, which hinders obtaining them in pure form. This study aims to develop a new, cheap and safe selective medium to isolate Pythium and Globisporangium species from their natural sources, despite the previous presence of more than 3 types of selective media including: VP3 (Vancomycin + PCNB + Penicillin + Pimaricin), NARM (Nystatin + Ampicillin + Rifampicin + Miconazole), and NARF (Nystatin + Ampicillin + Rifampicin + Fluazinam) used to isolate Oomycota. The elevated expense of antibiotics, along with alerts regarding potential contamination, toxicity, and tumor proliferation, renders them undesirable. Safe and inexpensive antibiotics from pharmaceutical sources were used with fixed formulations and concentrations. A selective medium was developed, called FANS (Fluconazole + Ampicillin + Nystatin + Sulbactam) with the following concentrations (1200 mg + 500 mg + 100,000 IU + 250 mg) respectively/L Corn Meal Agar promotes only the growth and formation of reproductive structures of Pythium and Globisporangium. On the other hand, these antibiotics completely inhibited the growth of other contaminated fungi and bacteria. Mycelial growth, zoospore formation, and oospore production for (Pythium aphanidermatum, Pythium oligandrum, and Globisporangium ultimum var. ultimum) were highest in FANS, followed by NARM, then NARF, while VP3 gave the lowest results compared to the control sample. It can be concluded that the use of FANS selective medium made the process of Pythium isolation easier, safer, and less expensive for worldwide researchers especially in developing and low-income countries.