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<p>Influenza viruses present significant challenges to global health. A rapid recombinase polymerase amplification (RPA)-based detection system for multiple influenza strains (A, H1N1/H2N2/H3N2/H5N1/H7N9 and B) has been developed to address the limitations of 2-h Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) tests. By using optimized primers targeting key viral proteins (M, NA, HA, PA), the method achieves detection in 10&#xa0;min with 0.99 log linearity (1–10<sup>6</sup> copies/μL). Clinical validation demonstrated 100% sensitivity and specificity, effectively distinguishing infections from healthy controls. This portable platform shows strong potential for point-of-care (POC) applications in resource-limited settings, offering timely diagnosis and epidemic control through its rapid and accurate detection capabilities.</p>

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Autonomous microfluidic influenza A/B subtyping system using on-chip multiplex isothermal amplification for field-deployable surveillance

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年8月21日

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  • Si-Ming Lu,
  • Jia-Long Wang,
  • Yang Li,
  • Ling Zhang,
  • Hang-Ping Yao,
  • Li-Guo Liang

摘要

Influenza viruses present significant challenges to global health. A rapid recombinase polymerase amplification (RPA)-based detection system for multiple influenza strains (A, H1N1/H2N2/H3N2/H5N1/H7N9 and B) has been developed to address the limitations of 2-h Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) tests. By using optimized primers targeting key viral proteins (M, NA, HA, PA), the method achieves detection in 10 min with 0.99 log linearity (1–106 copies/μL). Clinical validation demonstrated 100% sensitivity and specificity, effectively distinguishing infections from healthy controls. This portable platform shows strong potential for point-of-care (POC) applications in resource-limited settings, offering timely diagnosis and epidemic control through its rapid and accurate detection capabilities.