Scholarlink

<p>The pathogenesis of porcine epidemic diarrhea virus (PEDV) has not been fully clarified, which seriously hinders the prevention of the disease. The envelope (E) protein of PEDV induces the expression of pro-inflammatory cytokines, but the role of these inflammatory reactions in PEDV pathogenicity is still unknown. In this study, the asparagine at position 13 was found to be crucial to PEDV E protein induced inflammatory response. Exogenously expressing the parent E protein, rather than the E mutant carrying N13A, induces the activation of NF-κB and expression of inflammatory factors, including IL-6, IL-8, and TNF-α. Compared with the parental rPEDV strain, the&#xa0;recombinant strain&#xa0;rPEDV-E<sub>N13A</sub> exhibited a significantly lower infectious titer and formed smaller plaques. In addition, rPEDV-E<sub>N13A</sub> induced lower expression of inflammatory factors in vitro and in vivo. The pathogenicity assay shows that the rPEDV-E<sub>N13A</sub> strain caused diminished fecal PEDV RNA shedding, delayed death time, and milder histopathological lesions to intestinal villi. Our data provide a unique perspective for exploring the pathogenic mechanism of PEDV and a new target for the development of attenuated PEDV live vaccines.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The effect of asparagine-13 in porcine epidemic diarrhea virus envelope protein on pathogenicity

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年4月19日

查看全文
  • Zhiwei Li,
  • Zhiqian Ma,
  • Xiaojing Zhao,
  • Yongqi Li,
  • Congsen Zheng,
  • Yang Li,
  • Xuyang Guo,
  • Lele Xu,
  • Zifang Zheng,
  • Haixue Zheng,
  • Shuqi Xiao

摘要

The pathogenesis of porcine epidemic diarrhea virus (PEDV) has not been fully clarified, which seriously hinders the prevention of the disease. The envelope (E) protein of PEDV induces the expression of pro-inflammatory cytokines, but the role of these inflammatory reactions in PEDV pathogenicity is still unknown. In this study, the asparagine at position 13 was found to be crucial to PEDV E protein induced inflammatory response. Exogenously expressing the parent E protein, rather than the E mutant carrying N13A, induces the activation of NF-κB and expression of inflammatory factors, including IL-6, IL-8, and TNF-α. Compared with the parental rPEDV strain, the recombinant strain rPEDV-EN13A exhibited a significantly lower infectious titer and formed smaller plaques. In addition, rPEDV-EN13A induced lower expression of inflammatory factors in vitro and in vivo. The pathogenicity assay shows that the rPEDV-EN13A strain caused diminished fecal PEDV RNA shedding, delayed death time, and milder histopathological lesions to intestinal villi. Our data provide a unique perspective for exploring the pathogenic mechanism of PEDV and a new target for the development of attenuated PEDV live vaccines.