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<p>Pseudorabies virus (PRV) causes neurological disorders and organ damage in diseased animals. After initial infection, PRV activity is gradually inhibited; however, stress stimulation increases the host’s glucocorticoid levels, which overcomes the inhibition of PRV activity. Curcumin (Cur) helps maintain the inhibitory state of the Epstein-Barr virus, although further research is needed to establish whether Cur can prevent PRV activation triggered by stress hormones. In this study, we used PC-12 cells to determine the effects of Cur on PRV activation. The cells were successfully infected with PRV at a multiplicity of infection of 1 for 24&#xa0;h, resulting in the inhibition of PRV activity. Following incubation with 0.5&#xa0;µM dexamethasone (DEX) for 4&#xa0;h, the inhibition of PRV activity was blocked. Further mechanistic analyses using a dual-luciferase assay revealed that miR-155-5p directly targets and regulates Aak1 and its downstream signalling molecules, Numb and Notch2, in maintaining and disrupting PRV inhibition. Moreover, in vitro experiments using miR-155-5p mimics and inhibitors, combined with Aak1 overexpression and interference, confirmed that the miR-155-5p-Aak1-Numb/Notch2 axis prevented DEX-induced disruption of PRV inhibition by Cur. These findings provide a novel regulatory target for preventing stress-activated PRV and provide evidence for the potential use of Cur as a stress modulator in practical applications.</p>

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Curcumin prevents dexamethasone-induced activation of the pseudorabies virus in rat pheochromocytoma cells through the miR-155-5p-Aak1-Numb/Notch2 signalling axis

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月21日

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  • Naixiu Wang,
  • Fan Yang,
  • Zhiyun Qiu,
  • Lin Zhang,
  • Dingqiu Zou,
  • Yanru Tang,
  • Ruihan Zhang,
  • Chenlu Sun,
  • Pei Liu,
  • Kexin Qi,
  • Jingyi Wang,
  • Hua He,
  • Ling Gan

摘要

Pseudorabies virus (PRV) causes neurological disorders and organ damage in diseased animals. After initial infection, PRV activity is gradually inhibited; however, stress stimulation increases the host’s glucocorticoid levels, which overcomes the inhibition of PRV activity. Curcumin (Cur) helps maintain the inhibitory state of the Epstein-Barr virus, although further research is needed to establish whether Cur can prevent PRV activation triggered by stress hormones. In this study, we used PC-12 cells to determine the effects of Cur on PRV activation. The cells were successfully infected with PRV at a multiplicity of infection of 1 for 24 h, resulting in the inhibition of PRV activity. Following incubation with 0.5 µM dexamethasone (DEX) for 4 h, the inhibition of PRV activity was blocked. Further mechanistic analyses using a dual-luciferase assay revealed that miR-155-5p directly targets and regulates Aak1 and its downstream signalling molecules, Numb and Notch2, in maintaining and disrupting PRV inhibition. Moreover, in vitro experiments using miR-155-5p mimics and inhibitors, combined with Aak1 overexpression and interference, confirmed that the miR-155-5p-Aak1-Numb/Notch2 axis prevented DEX-induced disruption of PRV inhibition by Cur. These findings provide a novel regulatory target for preventing stress-activated PRV and provide evidence for the potential use of Cur as a stress modulator in practical applications.