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Background <p>Histone deacetylase 6 (HDAC6) is an enzyme pivotal for gene regulation, influencing cellular pathways through protein deacetylation. HDAC6 is a potential therapeutic target in diseases such as cancer and neurodegenerative disorders. Koole et al. investigated brain binding of [<sup>18</sup>F]Bavarostat, an HDAC6 inhibitor, in healthy participants, revealing an absolute test-retest variability (aTRV) of 7.7% (<i>n</i> = 4) for the distribution volume (<i>V</i><sub>T</sub>) with a 1-day interscan interval. This study aims to evaluate test-retest reproducibility with a more extended interscan interval.</p> Results <p>Six participants (3&#xa0;M/3F) underwent a test-retest scan, each lasting for 120&#xa0;min using a 4-ring Biograph mCT PET/CT scanner. Arterial blood sampling and metabolite analysis were performed to derive the input function. The two scans were 28 ± 12 days apart (14–43 days, <i>n</i> = 6). Regional time-activity curves (TACs) were generated for 15 regions of interest (ROIs). Kinetic analysis of the 120-min TACs was performed using one-tissue and two-tissue compartment models (1TC, 2TC) and multilinear analysis-1 (MA1) to quantify <i>V</i><sub>T</sub> values and compute absolute test-retest variability (aTRV). The effects of scan duration (60 to 120&#xa0;min) and MA1 <i>t</i>* setting on aTRV and bias were investigated. Careful analysis of the plasma HPLC data was needed since metabolites eluted close in time to the parent. The MA1 model (<i>t</i>* = 40&#xa0;min) adequately described regional TACs and produced stable kinetic parameters with good agreement to 2TC (MA1 <i>V</i><sub>T</sub>=0.98 × 2TC <i>V</i><sub>T</sub> + 0.48, bias: -0.1%) while 1TC underestimated <i>V</i><sub>T</sub> by 5.1%. Regional <i>V</i><sub>T</sub> values exhibited a relatively uniform pattern, highest in the amygdala and lowest in the centrum semiovale. Individual aTRV values ranged from 2 to 9%. Scan durations between 100 and 120&#xa0;min provided the most consistent results, with minimal bias and acceptable aTRV across all tested <i>t</i>* values. Although a 90-minute scan with <i>t</i>*=10 or 20-minute balanced scan time and aTRV, optimal parameters varied by brain region. Smaller regions (e.g., amygdala) required longer scans to achieve reliable <i>V</i><sub>T</sub> quantification.</p> Conclusions <p>The test-retest variability of [<sup>18</sup>F]Bavarostat <i>V</i><sub>T</sub> values demonstrated favorable results for a one-month scan interval, comparable to the reported values.</p>

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Assessment of test-retest reproducibility by [18F]Bavarostat for PET imaging of HDAC6

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月21日

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  • Mika Naganawa,
  • Ming-Qiang Zheng,
  • Jean-Dominique Gallezot,
  • Robin Bonomi,
  • Jiwei Gu,
  • Hong Gao,
  • Swanee Jacutin-Porte,
  • Nabeel B. Nabulsi,
  • Michel Koole,
  • Koen Van Laere,
  • David Matuskey,
  • Yiyun Huang,
  • Richard E. Carson

摘要

Background

Histone deacetylase 6 (HDAC6) is an enzyme pivotal for gene regulation, influencing cellular pathways through protein deacetylation. HDAC6 is a potential therapeutic target in diseases such as cancer and neurodegenerative disorders. Koole et al. investigated brain binding of [18F]Bavarostat, an HDAC6 inhibitor, in healthy participants, revealing an absolute test-retest variability (aTRV) of 7.7% (n = 4) for the distribution volume (VT) with a 1-day interscan interval. This study aims to evaluate test-retest reproducibility with a more extended interscan interval.

Results

Six participants (3 M/3F) underwent a test-retest scan, each lasting for 120 min using a 4-ring Biograph mCT PET/CT scanner. Arterial blood sampling and metabolite analysis were performed to derive the input function. The two scans were 28 ± 12 days apart (14–43 days, n = 6). Regional time-activity curves (TACs) were generated for 15 regions of interest (ROIs). Kinetic analysis of the 120-min TACs was performed using one-tissue and two-tissue compartment models (1TC, 2TC) and multilinear analysis-1 (MA1) to quantify VT values and compute absolute test-retest variability (aTRV). The effects of scan duration (60 to 120 min) and MA1 t* setting on aTRV and bias were investigated. Careful analysis of the plasma HPLC data was needed since metabolites eluted close in time to the parent. The MA1 model (t* = 40 min) adequately described regional TACs and produced stable kinetic parameters with good agreement to 2TC (MA1 VT=0.98 × 2TC VT + 0.48, bias: -0.1%) while 1TC underestimated VT by 5.1%. Regional VT values exhibited a relatively uniform pattern, highest in the amygdala and lowest in the centrum semiovale. Individual aTRV values ranged from 2 to 9%. Scan durations between 100 and 120 min provided the most consistent results, with minimal bias and acceptable aTRV across all tested t* values. Although a 90-minute scan with t*=10 or 20-minute balanced scan time and aTRV, optimal parameters varied by brain region. Smaller regions (e.g., amygdala) required longer scans to achieve reliable VT quantification.

Conclusions

The test-retest variability of [18F]Bavarostat VT values demonstrated favorable results for a one-month scan interval, comparable to the reported values.