Scholarlink

Background <p>Human mesenchymal stem cells (MSCs) are a promising stem cell source; however, their therapeutic efficacy in chronic wound healing remains limited. This study evaluates the therapeutic potential of transforming growth factor (TGF)-β1-modified, three-dimensionally cultured MSCs (A/T-3D) for enhancing wound healing. Methods: The <i>TGF-β1</i> gene was inserted into a safe genomic locus in adipose-derived MSCs (ASCs) using transcription activator-like effector nucleases. Quantitative polymerase chain reaction (qPCR) analysis showed that A/T-3D upregulated key factors related to wound healing, including epidermal growth factor (EGF), TGF-β1, fibroblast growth factor (FGF), and vascular endothelial growth factor (VEGF)-A, compared to unmodified ASCs. Results: In vitro scratch wound assays indicated that co-culture with A/T-3D-conditioned medium significantly accelerated wound closure in fibroblasts. In vivo, skin excision in nude mice treated with A/T-3D injections resulted in rapid wound closure, enhanced cellularity, and increased reepithelialization. High engraftment rates of A/T-3D and elevated expression of angiogenic factors were observed in the wound bed, highlighting their direct contribution to tissue repair. Conclusions: Collectively, these findings suggest that A/T-3D MSCs have significant therapeutic potential for wound healing through the secretion of epithelialization and angiogenic factors, along with enhanced engraftment capacity.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

3D TGF-β1-expressing mesenchymal stem cells enhance wound healing in a diabetic mouse model

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年9月2日

查看全文
  • Dong-Sik Chae,
  • Sang Joon An,
  • Seongho Han,
  • Sung-Whan Kim

摘要

Background

Human mesenchymal stem cells (MSCs) are a promising stem cell source; however, their therapeutic efficacy in chronic wound healing remains limited. This study evaluates the therapeutic potential of transforming growth factor (TGF)-β1-modified, three-dimensionally cultured MSCs (A/T-3D) for enhancing wound healing. Methods: The TGF-β1 gene was inserted into a safe genomic locus in adipose-derived MSCs (ASCs) using transcription activator-like effector nucleases. Quantitative polymerase chain reaction (qPCR) analysis showed that A/T-3D upregulated key factors related to wound healing, including epidermal growth factor (EGF), TGF-β1, fibroblast growth factor (FGF), and vascular endothelial growth factor (VEGF)-A, compared to unmodified ASCs. Results: In vitro scratch wound assays indicated that co-culture with A/T-3D-conditioned medium significantly accelerated wound closure in fibroblasts. In vivo, skin excision in nude mice treated with A/T-3D injections resulted in rapid wound closure, enhanced cellularity, and increased reepithelialization. High engraftment rates of A/T-3D and elevated expression of angiogenic factors were observed in the wound bed, highlighting their direct contribution to tissue repair. Conclusions: Collectively, these findings suggest that A/T-3D MSCs have significant therapeutic potential for wound healing through the secretion of epithelialization and angiogenic factors, along with enhanced engraftment capacity.