Scholarlink

Background <p>Bacteriocins are peptides that demonstrate antimicrobial activity and are produced by bacteria. <i>Bacillus velezensis</i> is considered a reliable producer of bacteriocins, with a strong antimicrobial spectrum and antimicrobial activity. In this study, <i>B. velezensis</i> G7 isolated from mangrove plant roots was used as the experimental strain. Whole-genome sequencing (WGS) analysis was performed to annotate functional genes.</p> Results <p>The results demonstrated that <i>B. velezensis</i> G7 has a genome size of 3,894,836&#xa0;bp, a GC content of 46.67%, and predicts 3,715 CDSs, 10 rRNA genes, and 83 tRNA genes. Gene annotation revealed pathways related to bacteriocin synthesis and immunity. Identified 8 bacteriocin and secondary metabolite biosynthetic gene clusters in <i>B. velezensis</i> G7, including Sactipeptides, LCI, Amylocyclicin, ComX4, Mersacidin, Lanthipeptide, Bacilysin, and NRPS. The crude protein from <i>B. velezensis</i> G7 shows broad-spectrum antibacterial activity, with the strongest inhibition against <i>Listeria monocytogenes</i> and the weakest against <i>Staphylococcus aureus</i>.</p> Conclusions <p>The genome of <i>B. velezensis</i> G7 was analyzed, and bacteriocin-related genes were identified. The results suggest that <i>B. velezensis</i> G7 has the potential to produce bacteriocins, thereby providing a theoretical basis for future studies on bacteriocin production in <i>B. velezensis</i>. In addition, these findings may facilitate further advances in bacteriocin research and bioinformatics analysis.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Whole genome sequencing and analysis of the bacteriocin-producing gene in Bacillus velezensis G7

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月29日

查看全文
  • Yang Li,
  • Haotian Ma,
  • Ruixue Pan,
  • Yuner Long,
  • Yining Zhao,
  • Jinju Peng,
  • Fucheng Guo,
  • Yuexia Ding,
  • Yi Ma

摘要

Background

Bacteriocins are peptides that demonstrate antimicrobial activity and are produced by bacteria. Bacillus velezensis is considered a reliable producer of bacteriocins, with a strong antimicrobial spectrum and antimicrobial activity. In this study, B. velezensis G7 isolated from mangrove plant roots was used as the experimental strain. Whole-genome sequencing (WGS) analysis was performed to annotate functional genes.

Results

The results demonstrated that B. velezensis G7 has a genome size of 3,894,836 bp, a GC content of 46.67%, and predicts 3,715 CDSs, 10 rRNA genes, and 83 tRNA genes. Gene annotation revealed pathways related to bacteriocin synthesis and immunity. Identified 8 bacteriocin and secondary metabolite biosynthetic gene clusters in B. velezensis G7, including Sactipeptides, LCI, Amylocyclicin, ComX4, Mersacidin, Lanthipeptide, Bacilysin, and NRPS. The crude protein from B. velezensis G7 shows broad-spectrum antibacterial activity, with the strongest inhibition against Listeria monocytogenes and the weakest against Staphylococcus aureus.

Conclusions

The genome of B. velezensis G7 was analyzed, and bacteriocin-related genes were identified. The results suggest that B. velezensis G7 has the potential to produce bacteriocins, thereby providing a theoretical basis for future studies on bacteriocin production in B. velezensis. In addition, these findings may facilitate further advances in bacteriocin research and bioinformatics analysis.